Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MARCKSL1 CRISPR/Cas9 KO Plasmid (h): sc-403985
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MARCKSL1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MARCKSL1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MARCKSL1 Antibody (K53): sc-130471
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MARCKSL1 CRISPR/Cas9 KO Plasmid (h) | sc-403985 | 20 µg | $397.00 |
Overview
MARCKSL1 (MARCKS-like 1) encodes an actin-binding, membrane-associated protein that regulates cytoskeletal remodeling, cell shape, and motility through phosphorylation-dependent interactions with phospholipids and the actin network. It functions in processes linked to neurite outgrowth, cell migration, and adhesion dynamics, integrating signals downstream of kinases such as PKC and pathways controlling lamellipodia formation. Altered MARCKSL1 expression has been associated with changes in invasive behavior and proliferation programs in multiple tumor contexts, and it is also studied in neurodevelopmental biology where actin reorganization is critical. These properties make MARCKSL1 a useful node for dissecting actin-driven cellular phenotypes and signaling-to-cytoskeleton coupling.
MARCKSL1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MARCKSL1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MARCKSL1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MARCKSL1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MARCKSL1 protein expression.
This CRISPR knockout system enables efficient generation of MARCKSL1-deficient cell models for investigation of MARCKSL1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MARCKSL1 exon(s) critical for MARCKSL1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MARCKSL1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MARCKSL1 CRISPR/Cas9 KO Plasmid (h) and MARCKSL1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MARCKSL1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MARCKSL1 HDR Plasmid (h) and MARCKSL1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MARCKSL1 homology arms to support homology-directed repair at defined MARCKSL1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.