MAP-2 Antibody (E-12): sc-74419

MAP-2 Antibody (E-12) is a mouse monoclonal IgG1 antibody that detects MAP-2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MAP-2 is a crucial microtubule-associated protein that plays a significant role in stabilizing microtubules and facilitating their assembly, which is essential for maintaining the structural integrity of neurons and supporting intracellular transport. MAP-2 is predominantly located in the dendrites of neurons, where MAP-2 contributes to the formation of dendritic spines and the overall architecture of the neuronal cytoskeleton. This localization is vital for proper synaptic function and plasticity, as MAP-2 allows for the dynamic remodeling of the cytoskeleton in response to neuronal activity. Furthermore, MAP-2 is subject to various post-translational modifications, including phosphorylation, which can regulate MAP-2′s interaction with microtubules and other proteins, thereby influencing neuronal development and response to injury. MAP-2 (E-12) antibody is an invaluable tool for researchers studying the dynamics of microtubule organization and the role of MAP-2 in neurodevelopmental and neurodegenerative processes.

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MAP-2 Antibody (E-12) References:

1. Melatonin attenuates the decrement of dendritic protein MAP-2 immuno-staining in the hippocampal CA1 and CA3 fields of the aging male rat.  |  Prieto-Gómez, B., et al. 2008. Neurosci Lett. 448: 56-61. PMID: 18951952
2. Motor Skill Training Promotes Sensorimotor Recovery and Increases Microtubule-Associated Protein-2 (MAP-2) Immunoreactivity in the Motor Cortex after Intracerebral Hemorrhage in the Rat.  |  Santos, MV., et al. 2013. ISRN Neurol. 2013: 159184. PMID: 23956876
3. Complexing of the CD-3 subunit by a monoclonal antibody activates a microtubule-associated protein 2 (MAP-2) serine kinase in Jurkat cells.  |  Hanekom, C., et al. 1989. Biochem J. 262: 449-56. PMID: 2552997
4. Multisite phosphorylation of microtubule-associated protein 2 (MAP-2) in rat brain: peptide mapping distinguishes between cyclic AMP-, calcium/calmodulin-, and calcium/phospholipid-regulated phosphorylation mechanisms.  |  Walaas, SI. and Nairn, AC. 1989. J Mol Neurosci. 1: 117-27. PMID: 2561875
5. Effects of adolescent methamphetamine and nicotine exposure on behavioral performance and MAP-2 immunoreactivity in the nucleus accumbens of adolescent mice.  |  Buck, JM., et al. 2017. Behav Brain Res. 323: 78-85. PMID: 28089854
6. An immunocytochemical and biochemical study of the microtubule-associated protein MAP-2 during post-lesion dendritic remodeling in the central nervous system of adult rats.  |  Cáceres, A., et al. 1988. Brain Res. 427: 233-46. PMID: 3042088
7. High molecular weight protein MAP 2 promoting microtubule assembly in vitro is associated with microtubules in cells.  |  Kuznetsov, SA., et al. 1980. Cell Biol Int Rep. 4: 1017-24. PMID: 7438221
8. Differential response of microtubule-associated protein 2 (MAP-2) in rat hippocampus after exposure to trimethyltin (TMT): an immunocytochemical study.  |  Koczyk, D. 1994. Acta Neurobiol Exp (Wars). 54: 55-8. PMID: 7517614
9. Characterization of the transcripts encoding two isoforms of human microtubule-associated protein-2 (MAP-2).  |  Albala, JS., et al. 1993. Gene. 136: 377-8. PMID: 8294038
10. Disruption of MAP-2 immunostaining in rat hippocampus after traumatic brain injury.  |  Folkerts, MM., et al. 1998. J Neurotrauma. 15: 349-63. PMID: 9605349

The preferred MAP-2 antibody is MAP-2 Antibody (A-4): sc-74421.