MAP-2 Antibody (A-4): sc-74421

MAP-2 Antibody (A-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects MAP-2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). MAP-2 (A-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MAP-2 protein plays a crucial role in stabilizing microtubules, which are essential components of the cytoskeleton, providing structural support and facilitating intracellular transport. Located primarily in neuronal cell bodies and dendrites, MAP-2 is vital for maintaining neuronal architecture integrity and regulating microtubule dynamics. MAP-2′s ability to bind tubulin and promote microtubule assembly is particularly important for neuronal development and function, helping ensure proper cell shape and signaling pathways. Additionally, MAP-2 undergoes various post-translational modifications, including phosphorylation, which can influence binding affinity and functional interactions with other proteins, thereby affecting microtubule stability and dynamics. anti-MAP-2 antibody (A-4)′s versatility in multiple applications makes MAP-2 (A-4) monoclonal antibody an invaluable tool for researchers studying cytoskeletal dynamics and neuronal function in various biological contexts.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

MAP-2 Antibody (A-4) References:

1. Towards an understanding of microtubule function and cell organization: an overview.  |  MacRae, TH. 1992. Biochem Cell Biol. 70: 835-41. PMID: 1297349
2. Immunochemical evidence that fragments of phosphorylated MAP5 (MAP1B) are bound to neurofibrillary tangles in Alzheimer's disease.  |  Hasegawa, M., et al. 1990. Neuron. 4: 909-18. PMID: 2361014
3. Numerous phosphates of microtubule-associated protein 2 in living rat brain.  |  Tsuyama, S., et al. 1987. J Biol Chem. 262: 10886-92. PMID: 3611094
4. Increased hepatic interleukin-1, arachidonic acid, and reactive oxygen species mediate the protective potential of peptides shared by gut cysteine peptidases against Schistosoma mansoni infection in mice.  |  Tallima, H. and El Ridi, R. 2023. PLoS Negl Trop Dis. 17: e0011164. PMID: 36920999
5. Interaction of microtubule-associated protein 2 with actin filaments.  |  Sattilaro, RF. 1986. Biochemistry. 25: 2003-9. PMID: 3707926
6. Role of tubulin-associated proteins in microtubule nucleation and elongation.  |  Murphy, DB., et al. 1977. J Mol Biol. 117: 33-52. PMID: 599568
7. Role of microtubule-associated proteins in the control of microtubule assembly.  |  Maccioni, RB. and Cambiazo, V. 1995. Physiol Rev. 75: 835-64. PMID: 7480164
8. Modulation of microtubule dynamic instability in vivo by brain microtubule associated proteins.  |  Dhamodharan, R. and Wadsworth, P. 1995. J Cell Sci. 108 (Pt 4): 1679-89. PMID: 7615685
9. The mitogen-activated protein kinase signal transduction pathway.  |  Davis, RJ. 1993. J Biol Chem. 268: 14553-6. PMID: 8325833
10. Differences in the regulation of microtubule dynamics by microtubule-associated proteins MAP1B and MAP2.  |  Vandecandelaere, A., et al. 1996. Cell Motil Cytoskeleton. 35: 134-46. PMID: 8894283
11. Cellular expression of MAP 2 kinase in rat brain.  |  Jacobowitz, DM., et al. 1996. Histochem Cell Biol. 106: 303-10. PMID: 8897071
12. Microtubule-associated proteins and the stimulation of tubulin assembly in vitro.  |  Sloboda, RD., et al. 1976. Biochemistry. 15: 4497-505. PMID: 974072

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MAP-2 Antibody (A-4) has 66 citations in a variety of scientific publications.

Primary antibodies like Anti-MAP-2 Antibody (A-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MAP-2 Antibody (A-4) Specifications

Alternate Product Name: MAP-2

Product Clone Name: A-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-300

Epitope Target: MAP-2

Epitope Species: human

Product Reactivity/Detection: MAP-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 66 citations in various scientific publications

Available Conjugates/Forms for MAP-2 Antibody (A-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin