Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MAN2C1 CRISPR/Cas9 KO Plasmid (h): sc-404066
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MAN2C1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MAN2C1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MAN2C1 Antibody (C-4): sc-377132
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MAN2C1 CRISPR/Cas9 KO Plasmid (h) | sc-404066 | 20 µg | $397.00 |
Overview
MAN2C1 encodes cytosolic alpha-mannosidase 2C1, a key enzyme in the catabolism of free N-glycans generated during glycoprotein turnover and quality control. By trimming mannose residues from oligosaccharides, MAN2C1 supports ER-associated degradation-adjacent glycan processing and contributes to cellular proteostasis and carbohydrate metabolism. Altered N-glycan handling and glycoprotein homeostasis have been linked to dysregulated signaling and metabolic adaptation in multiple disease contexts, making MAN2C1 a relevant node for studying glycan-dependent regulation of cell state. In human cells, perturbation of MAN2C1 can be used to interrogate how cytosolic glycan processing influences stress responses, lysosome–ER crosstalk, and glycoprotein-derived signaling networks.
MAN2C1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MAN2C1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MAN2C1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MAN2C1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MAN2C1 protein expression.
This CRISPR knockout system enables efficient generation of MAN2C1-deficient cell models for investigation of MAN2C1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MAN2C1 exon(s) critical for MAN2C1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MAN2C1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MAN2C1 CRISPR/Cas9 KO Plasmid (h) and MAN2C1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MAN2C1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MAN2C1 HDR Plasmid (h) and MAN2C1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MAN2C1 homology arms to support homology-directed repair at defined MAN2C1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.