MAN1B1 Antibody (E-10): sc-393145

MAN1B1 Antibody (E-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects MAN1B1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MAN1B1 (E-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MAN1B1 protein, also known as mannosidase alpha, class 1B, member 1, or MANA-ER, is a crucial enzyme belonging to the glycosyl hydrolase 47 family, primarily localized in the endoplasmic reticulum (ER). This single-pass type II membrane protein plays a vital role in the maturation of Asn-linked oligosaccharides on glycoproteins by catalyzing the initial mannose trimming step, which is essential for proper protein folding and function. MAN1B1′s significance extends to involvement in the endoplasmic reticulum-associated protein degradation (ERAD) pathway, targeting misfolded or unassembled glycoproteins for degradation via the ubiquitin-proteasome system, thereby maintaining cellular homeostasis. Additionally, MAN1B1 activity is calcium-dependent and can be inhibited by specific class I alpha-mannosidase inhibitors such as 1-deoxymannojirimycin and kifunensine, highlighting regulatory mechanisms in glycoprotein processing.

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MAN1B1 Antibody (E-10) References:

1. Identification, expression, and characterization of a cDNA encoding human endoplasmic reticulum mannosidase I, the enzyme that catalyzes the first mannose trimming step in mammalian Asn-linked oligosaccharide biosynthesis.  |  Gonzalez, DS., et al. 1999. J Biol Chem. 274: 21375-86. PMID: 10409699
2. Cloning and expression of a specific human alpha 1,2-mannosidase that trims Man9GlcNAc2 to Man8GlcNAc2 isomer B during N-glycan biosynthesis.  |  Tremblay, LO. and Herscovics, A. 1999. Glycobiology. 9: 1073-8. PMID: 10521544
3. Enhancement of endoplasmic reticulum (ER) degradation of misfolded Null Hong Kong alpha1-antitrypsin by human ER mannosidase I.  |  Hosokawa, N., et al. 2003. J Biol Chem. 278: 26287-94. PMID: 12736254
4. Elucidation of the molecular logic by which misfolded alpha 1-antitrypsin is preferentially selected for degradation.  |  Wu, Y., et al. 2003. Proc Natl Acad Sci U S A. 100: 8229-34. PMID: 12815101
5. Mechanism of class 1 (glycosylhydrolase family 47) {alpha}-mannosidases involved in N-glycan processing and endoplasmic reticulum quality control.  |  Karaveg, K., et al. 2005. J Biol Chem. 280: 16197-207. PMID: 15713668
6. Increased activity of lysosomal enzymes in the peritoneal fluid of patients with gynecologic cancers and pelvic inflammatory disease.  |  Beratis, NG., et al. 2005. J Cancer Res Clin Oncol. 131: 371-6. PMID: 15785934
7. Family 47 alpha-mannosidases in N-glycan processing.  |  Mast, SW. and Moremen, KW. 2006. Methods Enzymol. 415: 31-46. PMID: 17116466
8. Stimulation of ERAD of misfolded null Hong Kong alpha1-antitrypsin by Golgi alpha1,2-mannosidases.  |  Hosokawa, N., et al. 2007. Biochem Biophys Res Commun. 362: 626-32. PMID: 17727818
9. Diagnostic serum glycosylation profile in patients with intellectual disability as a result of MAN1B1 deficiency.  |  Van Scherpenzeel, M., et al. 2014. Brain. 137: 1030-8. PMID: 24566669
10. A Golgi-localized mannosidase (MAN1B1) plays a non-enzymatic gatekeeper role in protein biosynthetic quality control.  |  Iannotti, MJ., et al. 2014. J Biol Chem. 289: 11844-11858. PMID: 24627495
11. Somatic overgrowth associated with homozygous mutations in both MAN1B1 and SEC23A.  |  Gupta, S., et al. 2016. Cold Spring Harb Mol Case Stud. 2: a000737. PMID: 27148587
12. Case Report: Compound Heterozygous Variants of the MAN1B1 Gene in a Russian Patient with Rafiq Syndrome.  |  Zhalsanova, IZ., et al. 2022. Int J Mol Sci. 23: PMID: 36142510

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MAN1B1 Antibody (E-10) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-MAN1B1 Antibody (E-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MAN1B1 Antibody (E-10) Specifications

Alternate Product Name: MAN1B1

Product Clone Name: E-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 471-657

Epitope Target: MAN1B1

Epitope Species: human

Product Reactivity/Detection: MAN1B1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for MAN1B1 Antibody (E-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin