Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MALT1 CRISPR/Cas9 KO Plasmid (m): sc-433761
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MALT1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MALT1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MALT1 Antibody (D-1): sc-515389
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MALT1 CRISPR/Cas9 KO Plasmid (m) | sc-433761 | 20 µg | $397.00 |
Overview
Malt1 (MALT1) is a paracaspase and scaffold protein essential for antigen receptor–driven signaling in lymphocytes, functioning within the CARD11–BCL10–MALT1 (CBM) complex to couple receptor engagement to NF-κB and MAPK pathway activation. Through protease-dependent cleavage of multiple signaling regulators and protease-independent scaffolding, MALT1 helps tune transcriptional programs controlling immune cell activation, proliferation, and cytokine production. In mouse models, altered Malt1 activity perturbs immune homeostasis and is widely used to study mechanisms underlying inflammation, lymphocyte differentiation, and immune-mediated pathology. Its pathway connectivity also makes Malt1 a key node for dissecting signaling network rewiring in hematopoietic and immune cell contexts.
MALT1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Malt1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Malt1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Malt1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MALT1 protein expression.
This CRISPR knockout system enables efficient generation of Malt1-deficient cell models for investigation of MALT1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Malt1 exon(s) critical for MALT1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Malt1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MALT1 CRISPR/Cas9 KO Plasmid (m) and MALT1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Malt1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MALT1 HDR Plasmid (m) and MALT1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Malt1 homology arms to support homology-directed repair at defined Malt1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.