Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MAL CRISPR/Cas9 KO Plasmid (h): sc-403024
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MAL CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MAL genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MAL Antibody (E-1): sc-390687
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MAL CRISPR/Cas9 KO Plasmid (h) | sc-403024 | 20 µg | $397.00 |
Overview
MAL (myelin and lymphocyte protein) is a hydrophobic tetraspan membrane protein enriched in glycolipid- and cholesterol-rich membrane microdomains, where it contributes to apical membrane trafficking and stabilization of polarized epithelial architecture. It participates in vesicular sorting and transport processes that influence cell–cell junction organization and membrane protein distribution. MAL expression is frequently used as a marker of differentiation state in epithelial lineages and has been associated with altered membrane dynamics in multiple cancer contexts, including reports of epigenetic silencing or dysregulated expression linked to tumor progression and immune-related signaling. These properties make MAL a useful target for studying membrane compartmentalization, polarized transport, and phenotypes related to epithelial plasticity.
MAL CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MAL gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MAL together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MAL open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MAL protein expression.
This CRISPR knockout system enables efficient generation of MAL-deficient cell models for investigation of MAL signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MAL exon(s) critical for MAL function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MAL genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MAL CRISPR/Cas9 KO Plasmid (h) and MAL CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MAL locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MAL HDR Plasmid (h) and MAL HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MAL homology arms to support homology-directed repair at defined MAL target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.