MAGOH Antibody (F-6): sc-271365

MAGOH Antibody (F-6) is a mouse monoclonal IgG1 antibody that detects MAGOH in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MAGOH plays a vital role in embryo development and is ubiquitously expressed across various adult tissues, highlighting MAGOH′s importance in cellular functions. MAGOH features a unique structure characterized by an extremely flat, six-stranded anti-parallel β sheet adjacent to two helices, which is crucial for MAGOH′s interaction with Y14 protein. This interaction forms a complex essential for post-splicing processing, including nuclear export and cytoplasmic localization of mRNA, as well as the surveillance process known as nonsense-mediated mRNA decay (NMD). The MAGOH-Y14 complex remains associated with mRNA even after export to the cytoplasm, necessitating translation for eventual removal. This persistent association underscores MAGOH-Y14′s role in stabilizing the exon-exon junction complex-mRNA at splice junctions, thereby illustrating the intricate mechanisms of pre-mRNA splicing and implications for gene expression regulation.

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MAGOH Antibody (F-6) References:

1. MAGOH interacts with a novel RNA-binding protein.  |  Zhao, XF., et al. 2000. Genomics. 63: 145-8. PMID: 10662555
2. Structure of the Y14-Magoh core of the exon junction complex.  |  Lau, CK., et al. 2003. Curr Biol. 13: 933-41. PMID: 12781131
3. The EJC component Magoh regulates proliferation and expansion of neural crest-derived melanocytes.  |  Silver, DL., et al. 2013. Dev Biol. 375: 172-81. PMID: 23333945
4. Two mammalian MAGOH genes contribute to exon junction complex composition and nonsense-mediated decay.  |  Singh, KK., et al. 2013. RNA Biol. 10: 1291-8. PMID: 23917022
5. RNA-binding protein RBM8A (Y14) and MAGOH localize to centrosome in human A549 cells.  |  Ishigaki, Y., et al. 2014. Histochem Cell Biol. 141: 101-9. PMID: 23949737
6. The stability of Magoh and Y14 depends on their heterodimer formation and nuclear localization.  |  Ma, Q., et al. 2019. Biochem Biophys Res Commun. 511: 631-636. PMID: 30826064
7. MAGOH/MAGOHB Inhibits the Tumorigenesis of Gastric Cancer via Inactivation of b-RAF/MEK/ERK Signaling.  |  Zhou, Y., et al. 2020. Onco Targets Ther. 13: 12723-12735. PMID: 33328743
8. MAGOH and MAGOHB Knockdown in Melanoma Cells Decreases Nonsense-Mediated Decay Activity and Promotes Apoptosis via Upregulation of GADD45A.  |  Soederberg, A., et al. 2022. Cells. 11: PMID: 36497117
9. The paralogues MAGOH and MAGOHB are oncogenic factors in high-grade gliomas and safeguard the splicing of cell division and cell cycle genes.  |  Barreiro, RAS., et al. 2023. RNA Biol. 20: 311-322. PMID: 37294214
10. Upregulation of Long Noncoding RNA MAGOH-DT Mediates TNF-α and High Glucose-Induced Endothelial-Mesenchymal Transition in Arteriosclerosis Obliterans.  |  Wang, KJ., et al. 2024. Tohoku J Exp Med. 263: 227-238. PMID: 38811212

The preferred MAGOH antibody is MAGOH Antibody (21B12): sc-56724.