MAGOH Antibody (21B12): sc-56724
- MAGOH Antibody (21B12) is a mouse monoclonal IgG2a κ MAGOH antibody, cited in 8 publications, provided at 200 µg/ml
- raised against full length MAGOH of human origin
- MAGOH Antibody (21B12) is recommended for detection of MAGOH of mouse, rat and human origin by WB, IP, IF and IHC(P)
- Anti-MAGOH Antibody (21B12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MAGOH Antibody (21B12) for WB and IHC(P) applications. These reagents are now offered in bundles with MAGOH Antibody (21B12) (see ordering information below).
QUICK LINKS
SEE ALSO...
MAGOH Antibody (21B12) is a mouse monoclonal IgG2a kappa light chain antibody that detects MAGOH protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-MAGOH antibody (21B12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MAGOH, a human homolog of the Drosophila mago nashi, plays a vital role in embryo development and is ubiquitously expressed across adult tissues. MAGOH has a unique structure characterized by an extremely flat, six-stranded anti-parallel β sheet adjacent to two helices, which is essential for its function. This structural configuration facilitates the interaction between MAGOH and Y14 protein, forming a complex integral to post-splicing processing, including nuclear export and cytoplasmic localization of mRNA, as well as nonsense-mediated mRNA decay (NMD) surveillance process. MAGOH-Y14 complex persistence on mRNA after cytoplasmic export ensures stability of exon-exon junction complex-mRNA at splice junctions, demonstrating a key mechanism in pre-mRNA splicing machinery.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MAGOH Antibody (21B12) References:
- MAGOH interacts with a novel RNA-binding protein. | Zhao, XF., et al. 2000. Genomics. 63: 145-8. PMID: 10662555
- Magoh, a human homolog of Drosophila mago nashi protein, is a component of the splicing-dependent exon-exon junction complex. | Kataoka, N., et al. 2001. EMBO J. 20: 6424-33. PMID: 11707413
- Structure of the Y14-Magoh core of the exon junction complex. | Lau, CK., et al. 2003. Curr Biol. 13: 933-41. PMID: 12781131
- Splicing enhances translation in mammalian cells: an additional function of the exon junction complex. | Nott, A., et al. 2004. Genes Dev. 18: 210-22. PMID: 14752011
- An eIF4AIII-containing complex required for mRNA localization and nonsense-mediated mRNA decay. | Palacios, IM., et al. 2004. Nature. 427: 753-7. PMID: 14973490
- Association of the breast cancer protein MLN51 with the exon junction complex via its speckle localizer and RNA binding module. | Degot, S., et al. 2004. J Biol Chem. 279: 33702-15. PMID: 15166247
- A new function for nonsense-mediated mRNA-decay factors. | Wilkinson, MF. 2005. Trends Genet. 21: 143-8. PMID: 15734573
- Generation of a Magoh conditional allele in mice. | McMahon, JJ., et al. 2014. Genesis. 52: 752-8. PMID: 24771530
- Dosage-dependent requirements of Magoh for cortical interneuron generation and survival. | Sheehan, CJ., et al. 2020. Development. 147: PMID: 31857347
- MAGOH is correlated with poor prognosis and is essential for cell proliferation in lower-grade glioma. | Xiao, F., et al. 2023. Aging (Albany NY). 15: 5713-5733. PMID: 37390121
- MAGOH promotes gastric cancer progression via hnRNPA1 expression inhibition-mediated RONΔ160/PI3K/AKT signaling pathway activation. | Yu, S., et al. 2024. J Exp Clin Cancer Res. 43: 32. PMID: 38268030
- The mammalian homologue of mago nashi encodes a serum-inducible protein. | Zhao, XF., et al. 1998. Genomics. 47: 319-22. PMID: 9479507
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MAGOH Antibody (21B12) | sc-56724 | 200 µg/ml | $322.00 | |||
MAGOH Antibody (21B12): m-IgG Fc BP-HRP Bundle | sc-528619 | 200 µg Ab; 10 µg BP | $361.00 | |||
MAGOH Antibody (21B12): m-IgGκ BP-HRP Bundle | sc-521043 | 200 µg Ab, 40 µg BP | $361.00 | |||
MAGOH Antibody (21B12) AC | sc-56724 AC | 500 µg/ml, 25% agarose | $424.00 | |||
MAGOH Antibody (21B12) HRP | sc-56724 HRP | 200 µg/ml | $322.00 | |||
MAGOH Antibody (21B12) FITC | sc-56724 FITC | 200 µg/ml | $336.00 | |||
MAGOH Antibody (21B12) PE | sc-56724 PE | 200 µg/ml | $349.00 | |||
MAGOH Antibody (21B12) Alexa Fluor® 488 | sc-56724 AF488 | 200 µg/ml | $364.00 | |||
MAGOH Antibody (21B12) Alexa Fluor® 546 | sc-56724 AF546 | 200 µg/ml | $364.00 | |||
MAGOH Antibody (21B12) Alexa Fluor® 594 | sc-56724 AF594 | 200 µg/ml | $364.00 | |||
MAGOH Antibody (21B12) Alexa Fluor® 647 | sc-56724 AF647 | 200 µg/ml | $364.00 | |||
MAGOH Antibody (21B12) Alexa Fluor® 680 | sc-56724 AF680 | 200 µg/ml | $364.00 | |||
MAGOH Antibody (21B12) Alexa Fluor® 790 | sc-56724 AF790 | 200 µg/ml | $364.00 |