Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MAGI-1 CRISPR/Cas9 KO Plasmid (h): sc-402283
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MAGI-1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MAGI-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MAGI-1 Antibody (SS-5): sc-100326
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MAGI-1 CRISPR/Cas9 KO Plasmid (h) | sc-402283 | 20 µg | $397.00 |
Overview
MAGI1 encodes MAGI-1, a membrane-associated guanylate kinase (MAGUK) scaffold protein enriched at tight junctions and other cell–cell contact sites where it organizes multiprotein signaling complexes. By binding junctional components and signaling effectors through PDZ and WW domains, MAGI-1 helps coordinate epithelial polarity, adherens/tight junction stability, and spatial regulation of pathways linked to cytoskeletal remodeling and signal transduction. Altered MAGI1 expression or junctional localization has been associated with disruption of barrier function and aberrant cell migration programs, processes frequently studied in cancer biology and inflammatory tissue remodeling. MAGI-1 is also used as a node to interrogate cross-talk between junctional architecture and growth factor–responsive signaling networks.
MAGI-1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MAGI1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MAGI1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MAGI1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MAGI-1 protein expression.
This CRISPR knockout system enables efficient generation of MAGI1-deficient cell models for investigation of MAGI-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MAGI1 exon(s) critical for MAGI-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MAGI1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MAGI-1 CRISPR/Cas9 KO Plasmid (h) and MAGI-1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MAGI1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MAGI-1 HDR Plasmid (h) and MAGI-1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MAGI1 homology arms to support homology-directed repair at defined MAGI1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.