MAG Antibody (A-11): sc-166849

MAG Antibody (A-11) is a mouse monoclonal IgG2a kappa light chain antibody that detects MAG protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). MAG Antibody (A-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Myelin-associated glycoprotein (MAG) plays a crucial role in the nervous system as a cell-surface adhesion protein that facilitates the connection between myelinating glial cells and neuronal axons. MAG is characterized by a sialic acid binding site and five immunoglobulin-like domains, classifying MAG within a specific subgroup of the immunoglobulin superfamily. This unique structure aids in the adhesion process and contributes to the inhibitory properties of myelin, which can impede axonal growth. Growth inhibition mediated by MAG can be counteracted when cerebellar neurons are pre-treated with neurotrophins such as brain-derived neurotrophic factor (BDNF) or glial cell line-derived neurotrophic factor (GDNF), suggesting these neurotrophins may enhance cellular signaling pathways, including the elevation of cyclic adenosine monophosphate (cAMP) and the activation of protein kinase A (PKA).

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

MAG Antibody (A-11) References:

1. Prior exposure to neurotrophins blocks inhibition of axonal regeneration by MAG and myelin via a cAMP-dependent mechanism.  |  Cai, D., et al. 1999. Neuron. 22: 89-101. PMID: 10027292
2. Multiple functions of the myelin-associated glycoprotein MAG (siglec-4a) in formation and maintenance of myelin.  |  Schachner, M. and Bartsch, U. 2000. Glia. 29: 154-65. PMID: 10625334
3. Gangliosides are functional nerve cell ligands for myelin-associated glycoprotein (MAG), an inhibitor of nerve regeneration.  |  Vyas, AA., et al. 2002. Proc Natl Acad Sci U S A. 99: 8412-7. PMID: 12060784
4. Degraded myelin-associated glycoprotein (dMAG) formation from pure human brain myelin-associated glycoprotein (MAG) is not mediated by calpain or cathepsin L-like activities.  |  Päiväläinen, S., et al. 2003. J Neurochem. 84: 533-45. PMID: 12558973
5. Nogo-66 and myelin-associated glycoprotein (MAG) inhibit the adhesion and migration of Nogo-66 receptor expressing human glioma cells.  |  Liao, H., et al. 2004. J Neurochem. 90: 1156-62. PMID: 15312170
6. Neural protein 1B236/myelin-associated glycoprotein (MAG) defines a subgroup of the immunoglobulin superfamily.  |  Lai, C., et al. 1987. Immunol Rev. 100: 129-51. PMID: 2450062
7. Myelin-associated glycoprotein demonstrated immunocytochemically in myelin and myelin-forming cells of developing rat.  |  Sternberger, NH., et al. 1979. Proc Natl Acad Sci U S A. 76: 1510-4. PMID: 375239
8. Identification of myelin-associated glycoprotein as a major myelin-derived inhibitor of neurite growth.  |  McKerracher, L., et al. 1994. Neuron. 13: 805-11. PMID: 7524558
9. Sialic acid specificity of myelin-associated glycoprotein binding.  |  Collins, BE., et al. 1997. J Biol Chem. 272: 1248-55. PMID: 8995428
10. Glycoproteins of myelin sheaths.  |  Quarles, RH. 1997. J Mol Neurosci. 8: 1-12. PMID: 9061610
11. Myelin-associated glycoprotein is a myelin signal that modulates the caliber of myelinated axons.  |  Yin, X., et al. 1998. J Neurosci. 18: 1953-62. PMID: 9482781
12. Myelin-associated glycoprotein in myelin and expressed by Schwann cells inhibits axonal regeneration and branching.  |  Shen, YJ., et al. 1998. Mol Cell Neurosci. 12: 79-91. PMID: 9770342

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MAG Antibody (A-11) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-MAG Antibody (A-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MAG Antibody (A-11) Specifications

Alternate Product Name: Myelin Associated Glycoprotein

Product Clone Name: A-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-300

Epitope Target: MAG

Epitope Species: human

Product Reactivity/Detection: MAG

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for MAG Antibody (A-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin