MafB Antibody (F-11): sc-74521

MafB Antibody (F-11) is a mouse monoclonal IgG2a kappa light chain antibody that detects MafB protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-MafB antibody (F-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MafB, a member of the basic region/leucine zipper (bZIP) transcription factor family, plays a crucial role in regulating gene expression during hematopoiesis, particularly by repressing Ets-1-mediated transcription of key erythroid-specific genes in myeloid cells. This regulation is vital for proper blood cell development and differentiation, as it ensures that the expression of genes necessary for erythroid lineage is tightly controlled. MafB is expressed across various tissues and contains a characteristic bZIP motif in its carboxy-terminal region, which facilitates its function as a transcriptional activator. Additionally, MafB interacts with other transcription factors, such as c-Myb, to form complexes that mediate cooperative interactions essential for early myeloid cell differentiation. The ability of MafB to modulate transcriptional activity based on its protein partners highlights its importance in developmental processes and cellular responses, making MafB (F-11) monoclonal antibody an invaluable tool for researchers studying hematopoiesis and related pathways.

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MafB Antibody (F-11) References:

1. Regulation of mouse lens fiber cell development and differentiation by the Maf gene.  |  Ring, BZ., et al. 2000. Development. 127: 307-17. PMID: 10603348
2. Identification of primary MAFB target genes in multiple myeloma.  |  van Stralen, E., et al. 2009. Exp Hematol. 37: 78-86. PMID: 19013005
3. MafB is a critical regulator of complement component C1q.  |  Tran, MTN., et al. 2017. Nat Commun. 8: 1700. PMID: 29167450
4. Transcription factor MafB is a marker of tumor-associated macrophages in both mouse and humans.  |  Yadav, MK., et al. 2020. Biochem Biophys Res Commun. 521: 590-595. PMID: 31679694
5. Transcription Factor MAFB as a Prognostic Biomarker for the Lung Adenocarcinoma.  |  Samir, O., et al. 2022. Int J Mol Sci. 23: PMID: 36077342
6. MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.  |  Yadav, MK., et al. 2024. Cell Rep. 43: 113978. PMID: 38522069
7. Conditional expression of the ubiquitous transcription factor MafK induces erythroleukemia cell differentiation.  |  Igarashi, K., et al. 1995. Proc Natl Acad Sci U S A. 92: 7445-9. PMID: 7638211
8. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor.  |  Kataoka, K., et al. 1995. Mol Cell Biol. 15: 2180-90. PMID: 7891713
9. A conserved region adjacent to the basic domain is required for recognition of an extended DNA binding site by Maf/Nrl family proteins.  |  Kerppola, TK. and Curran, T. 1994. Oncogene. 9: 3149-58. PMID: 7936637
10. Small Maf proteins interact with the human transcription factor TCF11/Nrf1/LCR-F1.  |  Johnsen, O., et al. 1996. Nucleic Acids Res. 24: 4289-97. PMID: 8932385
11. c-Maf interacts with c-Myb to regulate transcription of an early myeloid gene during differentiation.  |  Hedge, SP., et al. 1998. Mol Cell Biol. 18: 2729-37. PMID: 9566892
12. Rat maf-related factors: the specificities of DNA binding and heterodimer formation.  |  Matsushima-Hibiya, Y., et al. 1998. Biochem Biophys Res Commun. 245: 412-8. PMID: 9571165

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MafB Antibody (F-11) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-MafB Antibody (F-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MafB Antibody (F-11) Specifications

Alternate Product Name: Transcription Factor MafB

Product Clone Name: F-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 111-210

Epitope Target: MafB

Epitope Species: human

Product Reactivity/Detection: MafB

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for MafB Antibody (F-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent