MAD2 Antibody (107-276-3): sc-65492

MAD2 Antibody (107-276-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects MAD2 in mouse, rat, and human samples through applications such as immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MAD2 antibody (107-276-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MAD2 plays a critical role in regulating the cell cycle, specifically at the mitotic spindle assembly checkpoint, ensuring proper chromosome segregation during cell division. By binding to unattached kinetochores during prometaphase, MAD2 prevents progression to anaphase until all chromosomes are correctly aligned and attached to the spindle apparatus. This function is vital for maintaining genomic stability, as cells lacking functional MAD2 can experience missegregation of chromosomes, leading to aneuploidy and potential tumorigenesis. Studies have shown that breast carcinoma cell lines with diminished MAD2 expression, such as T47D, can bypass the mitotic checkpoint even in the presence of nocodazole, a drug that disrupts spindle assembly, highlighting MAD2′s importance in cancer biology. MAD2 has been implicated in specific signaling pathways, associating with the insulin receptor, suggesting a role beyond cell cycle regulation. The presence of MAD2B, a MAD2 homolog, further emphasizes the mitotic checkpoint′s complexity, as MAD2B is essential for monitoring kinetochore-spindle attachment and delaying anaphase onset if the attachment is incomplete.

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MAD2 Antibody (107-276-3) References:

1. Activation of MAD 2 checkprotein and persistence of cyclin B1/CDC 2 activity associate with paclitaxel-induced apoptosis in human nasopharyngeal carcinoma cells.  |  Huang, TS., et al. 2000. Apoptosis. 5: 235-41. PMID: 11225845
2. Structural activation of Mad2 in the mitotic spindle checkpoint: the two-state Mad2 model versus the Mad2 template model.  |  Yu, H. 2006. J Cell Biol. 173: 153-7. PMID: 16636141
3. The Mad2 partial unfolding model: regulating mitosis through Mad2 conformational switching.  |  Skinner, JJ., et al. 2008. J Cell Biol. 183: 761-8. PMID: 19029339
4. The Mad1-Mad2 balancing act--a damaged spindle checkpoint in chromosome instability and cancer.  |  Schuyler, SC., et al. 2012. J Cell Sci. 125: 4197-206. PMID: 23093575
5. Structure of an intermediate conformer of the spindle checkpoint protein Mad2.  |  Hara, M., et al. 2015. Proc Natl Acad Sci U S A. 112: 11252-7. PMID: 26305957
6. Conformation-specific anti-Mad2 monoclonal antibodies for the dissection of checkpoint signaling.  |  Sedgwick, GG., et al. 2016. MAbs. 8: 689-97. PMID: 26986935
7. The role of the MAD2-TLR4-MyD88 axis in paclitaxel resistance in ovarian cancer.  |  Bates, M., et al. 2020. PLoS One. 15: e0243715. PMID: 33370338
8. The Mad2-Binding Protein p31comet as a Potential Target for Human Cancer Therapy.  |  Henriques, AC., et al. 2021. Curr Cancer Drug Targets. 21: 401-415. PMID: 33511944
9. CAMK2D serves as a molecular scaffold for RNF8-MAD2 complex to induce mitotic checkpoint in glioma.  |  Chuah, YH., et al. 2023. Cell Death Differ. 30: 1973-1987. PMID: 37468549
10. Mad2 is dispensable for accurate chromosome segregation but becomes essential when oocytes are subjected to environmental stress.  |  Qiao, JY., et al. 2023. Development. 150: PMID: 37485540

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MAD2 Antibody (107-276-3) has 16 citations in a variety of scientific publications.

Primary antibodies like Anti-MAD2 Antibody (107-276-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MAD2 Antibody (107-276-3) Specifications

Alternate Product Name: MAD2L1

Product Clone Name: 107-276-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: MAD2

Epitope Species: human

Product Reactivity/Detection: MAD2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 16 citations in various scientific publications

Available Conjugates/Forms for MAD2 Antibody (107-276-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred MAD2 antibody is MAD2 Antibody (17D10): sc-47747.