MAD1 Antibody (C-5): sc-137025

MAD1 Antibody (C-5) is a mouse monoclonal IgG1 antibody that detects MAD1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MAD1 is a critical component of the mitotic spindle assembly checkpoint, ensuring that cell cycle progression is halted until all chromosomes are properly aligned at the metaphase plate, which is vital for maintaining genomic stability. MAD1 primarily localizes to the nucleus, where MAD1 plays a key role in anchoring MAD2L1 to the nuclear periphery, but during mitosis, MAD1 dynamically relocates to the centrosome, spindle midzone, and midbody, highlighting MAD1′s importance in the spatial regulation of cell division. MAD1 can form homo- and heterodimers, contributing to the formation of the tetrameric MAD1L1-MAD2L1 core complex, which is essential for MAD1′s function. Notably, MAD1 is subject to hyperphosphorylation in response to mitotic spindle damage, a modification that enhances MAD1′s activity and underscores MAD1′s role in the cellular response to stress. Defects in the gene encoding MAD1, MAD1L1, are implicated in the development and progression of various cancers, making anti-MAD1 antibody (C-5) a valuable tool for research into cell cycle regulation and cancer biology.

The preferred MAD1 antibody is MAD1 Antibody (9B10): sc-47746.