MAD1 Antibody (9B10): sc-47746

MAD1 Antibody (9B10) is a mouse monoclonal IgG2b kappa light chain antibody that detects MAD1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). MAD1 (9B10) antibody is available in both conjugated and non-conjugated forms, providing flexibility for various experimental needs. MAD1 protein plays a critical role in cell cycle regulation, particularly at the mitotic spindle assembly checkpoint, where MAD1 ensures cell division proceeds only when all chromosomes are properly aligned. This function is vital for maintaining genomic stability, as errors in chromosome segregation can lead to aneuploidy and contribute to cancer development. MAD1 interacts with MAD2 to form a tetrameric core complex, which is essential for the checkpoint′s function. MAD1′s localization is dynamic; MAD1 primarily resides in the nucleus but translocates to the centrosome and spindle midzone during mitosis, highlighting MAD1′s importance in coordinating cell division. MAD1 undergoes hyperphosphorylation in response to spindle damage, a modification that enhances MAD1′s activity and underscores MAD1′s role in cellular stress responses. Defects in MAD1 have been implicated in various cancers, making MAD1 a significant target for research in cancer biology and therapeutic development.

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MAD1 Antibody (9B10) References:

1. Expression of Mad1 protein inhibits proliferation of cancer cells and inversely correlated with Myc protein expression in primary gastric cancer.  |  Han, S., et al. 1999. Oncol Rep. 6: 569-74. PMID: 10203593
2. Myc family oncoproteins function through a common pathway to transform normal cells in culture: cross-interference by Max and trans-acting dominant mutants.  |  Mukherjee, B., et al. 1992. Genes Dev. 6: 1480-92. PMID: 1644290
3. Association of Myn, the murine homolog of max, with c-Myc stimulates methylation-sensitive DNA binding and ras cotransformation.  |  Prendergast, GC., et al. 1991. Cell. 65: 395-407. PMID: 1840505
4. Intracellular leucine zipper interactions suggest c-Myc hetero-oligomerization.  |  Dang, CV., et al. 1991. Mol Cell Biol. 11: 954-62. PMID: 1990293
5. Max: a helix-loop-helix zipper protein that forms a sequence-specific DNA-binding complex with Myc.  |  Blackwood, EM. and Eisenman, RN. 1991. Science. 251: 1211-7. PMID: 2006410
6. Transcriptional regulation by dimerization: two sides to an incestuous relationship.  |  Jones, N. 1990. Cell. 61: 9-11. PMID: 2180585
7. Mad1 mediates hypoxia-induced doxorubicin resistance in colon cancer cells by inhibiting mitochondrial function.  |  Cho, K., et al. 2013. Free Radic Biol Med. 60: 201-10. PMID: 23459071
8. Alterations of DNA damage response pathway: Biomarker and therapeutic strategy for cancer immunotherapy.  |  Jiang, M., et al. 2021. Acta Pharm Sin B. 11: 2983-2994. PMID: 34729299
9. The Sin3A/MAD1 Complex, through Its PAH2 Domain, Acts as a Second Repressor of Retinoic Acid Receptor Beta Expression in Breast Cancer Cells.  |  Dahiya, NR., et al. 2022. Cells. 11: PMID: 35406744
10. Mad: a heterodimeric partner for Max that antagonizes Myc transcriptional activity.  |  Ayer, DE., et al. 1993. Cell. 72: 211-22. PMID: 8425218
11. Mxi1, a protein that specifically interacts with Max to bind Myc-Max recognition sites.  |  Zervos, AS., et al. 1993. Cell. 72: 223-32. PMID: 8425219
12. Oncogenic activity of the c-Myc protein requires dimerization with Max.  |  Amati, B., et al. 1993. Cell. 72: 233-45. PMID: 8425220

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MAD1 Antibody (9B10) has 21 citations in a variety of scientific publications.

Primary antibodies like Anti-MAD1 Antibody (9B10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MAD1 Antibody (9B10) Specifications

Alternate Product Name: MAD1

Product Clone Name: 9B10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids:

Epitope Target: MAD1

Epitope Species: human

Product Reactivity/Detection: MAD1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 21 citations in various scientific publications

Available Conjugates/Forms for MAD1 Antibody (9B10): AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, PE: Phycoerythrin