mAChR M3 Antibody (H-4): sc-518107

mAChR M3 Antibody (H-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects mAChR M3 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-mAChR M3 antibody (H-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The muscarinic acetylcholine receptor M3 (mAChR M3) plays a crucial role in mediating diverse cellular responses, such as the inhibition of adenylate cyclase and the modulation of ion channels, which are essential for maintaining cellular homeostasis and facilitating communication between cells. Located primarily in the central nervous system and various peripheral tissues, mAChR M3 is involved in numerous physiological processes, including smooth muscle contraction and glandular secretion. mAChR M3′s unique structure, characterized by seven transmembrane domains typical of G-protein coupled receptors, allows effective signal transduction through G-proteins, leading to the activation of downstream effector proteins. The distinct tissue distribution and functional properties of mAChR M3 highlight its importance in pharmacological research and therapeutic applications, making mAChR M3 (H-4) monoclonal antibody an invaluable tool for studying this receptor′s role in health and disease.

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mAChR M3 Antibody (H-4) References:

1. Muscarinic acetylcholine receptors: structure and function.  |  Hulme, EC., et al. 1991. Biochem Soc Trans. 19: 133-8. PMID: 1903735
2. Muscarinic acetylcholine receptors: typical G-coupled receptors.  |  Hulme, EC. 1990. Symp Soc Exp Biol. 44: 39-54. PMID: 2130518
3. M3 mAChR-mediated IL-8 expression through PKC/NF-κB signaling pathways.  |  Xu, ZP., et al. 2014. Inflamm Res. 63: 463-73. PMID: 24522860
4. Molecular cloning and expression of a fifth muscarinic acetylcholine receptor.  |  Liao, CF., et al. 1989. J Biol Chem. 264: 7328-37. PMID: 2540186
5. Primary structure and biochemical properties of an M2 muscarinic receptor.  |  Peralta, EG., et al. 1987. Science. 236: 600-5. PMID: 3107123
6. Huperzine A lowers intraocular pressure via the M3 mAChR and provides retinal neuroprotection via the M1 mAChR: a promising agent for the treatment of glaucoma.  |  Yu, P., et al. 2021. Ann Transl Med. 9: 332. PMID: 33708959
7. PD 102807 Induces M3 mAChR-Dependent GRK-/Arrestin-Biased Signaling in Airway Smooth Muscle Cells.  |  Tompkins, E., et al. 2022. Am J Respir Cell Mol Biol. 67: 550-561. PMID: 35944139
8. The biased M3 mAChR ligand PD 102807 mediates qualitatively distinct signaling to regulate airway smooth muscle phenotype.  |  Tompkins, E., et al. 2023. J Biol Chem. 299: 105209. PMID: 37660916
9. Muscarinic receptors--characterization, coupling and function.  |  Caulfield, MP. 1993. Pharmacol Ther. 58: 319-79. PMID: 7504306
10. Muscarinic acetylcholine receptor subtypes: localization and structure/function.  |  Brann, MR., et al. 1993. Prog Brain Res. 98: 121-7. PMID: 8248499
11. Distribution of muscarinic receptor subtypes in rat brain from postnatal to old age.  |  Tice, MA., et al. 1996. Brain Res Dev Brain Res. 92: 70-6. PMID: 8861724
12. Pharmacology of muscarinic acetylcholine receptor subtypes (m1-m5): high throughput assays in mammalian cells.  |  Bräuner-Osborne, H. and Brann, MR. 1996. Eur J Pharmacol. 295: 93-102. PMID: 8925880

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-mAChR M3 Antibody (H-4) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-mAChR M3 Antibody (H-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

mAChR M3 Antibody (H-4) Specifications

Alternate Product Name: mAChR M3

Product Clone Name: H-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 562-590

Epitope Target: mAChR M3

Epitope Species: human

Product Reactivity/Detection: mAChR M3

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for mAChR M3 Antibody (H-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin