MACF1 CRISPR/Cas9 KO Plasmid (m): sc-418945
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MACF1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MACF1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MACF1 Antibody (A-3): sc-377532
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MACF1 CRISPR/Cas9 KO Plasmid (m) | sc-418945 | 20 µg | $397.00 |
Overview
Macf1 encodes microtubule actin crosslinking factor 1 (MACF1), a large spectraplakin that mechanically and functionally links microtubules with the actin cytoskeleton to coordinate cell polarity, migration, and intracellular trafficking. In mouse cells, MACF1 supports focal adhesion turnover and cytoskeletal remodeling and has been connected to Wnt/β-catenin signaling through regulation of Axin complex dynamics, influencing cytoskeleton-dependent signaling outputs. By integrating actin and microtubule networks, MACF1 contributes to neuronal development, epidermal and intestinal tissue organization, and responses to mechanical cues. Dysregulation of MACF1-associated cytoskeletal programs has been studied in contexts such as neurodevelopmental phenotypes, barrier integrity, and metastatic-like migration behaviors relevant to cancer biology models.
MACF1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Macf1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Macf1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Macf1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MACF1 protein expression.
This CRISPR knockout system enables efficient generation of Macf1-deficient cell models for investigation of MACF1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Macf1 exon(s) critical for MACF1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Macf1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MACF1 CRISPR/Cas9 KO Plasmid (m) and MACF1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Macf1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MACF1 HDR Plasmid (m) and MACF1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Macf1 homology arms to support homology-directed repair at defined Macf1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.