MACF1 Antibody (A-3): sc-377532
- MACF1 Antibody (A-3) is a mouse monoclonal IgG1 κ MACF1 antibody, cited in 4 publications, provided at 200 µg/ml
- raised against amino acids 1997-2240 mapping within an internal region of MACF1 of mouse origin
- MACF1 Antibody (A-3) is recommended for detection of MACF1 of mouse and rat origin by WB, IP, IF and ELISA
- Anti-MACF1 Antibody (A-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MACF1 Antibody (A-3) for WB applications. These reagents are now offered in bundles with MACF1 Antibody (A-3) (see ordering information below).
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MACF1 Antibody (A-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects MACF1 of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MACF1 (A-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MACF1, or Microtubule-actin cross-linking factor 1, is a large 5,327 amino acid protein encoded by the human MACF1 gene, belonging to the plakin or cytolinker family. MACF1 plays a crucial role in maintaining cellular architecture by cross-linking actin filaments to microtubules, which is essential for cellular stability and integrity. The presence of one actin-binding domain, two calponin-homology domains, two EF-hand domains, one SH3 domain, and thirty-seven spectrin repeats in MACF1′s structure allows interaction with various cytoskeletal components, facilitating the organization of the cytoskeleton in response to mechanical stress. MACF1 is predominantly expressed in the cytoplasm of tissues such as the lung, brain, spinal cord, skeletal and cardiac muscle, and skin, highlighting MACF1′s importance in both structural support and cellular signaling pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MACF1 Antibody (A-3) References:
- Interaction between p230 and MACF1 is associated with transport of a glycosyl phosphatidyl inositol-anchored protein from the Golgi to the cell periphery. | Kakinuma, T., et al. 2004. Exp Cell Res. 298: 388-98. PMID: 15265687
- Microtubule actin crosslinking factor 1b: a novel plakin that localizes to the Golgi complex. | Lin, CM., et al. 2005. J Cell Sci. 118: 3727-38. PMID: 16076900
- Comprehensive identification of phosphorylation sites in postsynaptic density preparations. | Trinidad, JC., et al. 2006. Mol Cell Proteomics. 5: 914-22. PMID: 16452087
- The role of microtubule actin cross-linking factor 1 (MACF1) in the Wnt signaling pathway. | Chen, HJ., et al. 2006. Genes Dev. 20: 1933-45. PMID: 16815997
- Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations. | Munton, RP., et al. 2007. Mol Cell Proteomics. 6: 283-93. PMID: 17114649
- Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry. | Dai, J., et al. 2007. J Proteome Res. 6: 250-62. PMID: 17203969
- Large-scale phosphorylation analysis of mouse liver. | Villén, J., et al. 2007. Proc Natl Acad Sci U S A. 104: 1488-93. PMID: 17242355
- Isoforms, structures, and functions of versatile spectraplakin MACF1. | Hu, L., et al. 2016. BMB Rep. 49: 37-44. PMID: 26521939
- MACF1, versatility in tissue-specific function and in human disease. | Hu, L., et al. 2017. Semin Cell Dev Biol. 69: 3-8. PMID: 28577926
- The role of MACF1 in nervous system development and maintenance. | Moffat, JJ., et al. 2017. Semin Cell Dev Biol. 69: 9-17. PMID: 28579452
- MACF1 alleviates aging-related osteoporosis via HES1. | Yin, C., et al. 2021. J Cell Mol Med. 25: 6242-6257. PMID: 34133068
- MACF1 overexpression in BMSCs alleviates senile osteoporosis in mice through TCF4/miR-335-5p signaling pathway. | Zhang, K., et al. 2023. J Orthop Translat. 39: 177-190. PMID: 36969134
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MACF1 Antibody (A-3) | sc-377532 | 200 µg/ml | $322.00 | |||
MACF1 Antibody (A-3): m-IgG Fc BP-HRP Bundle | sc-530037 | 200 µg Ab; 10 µg BP | $361.00 | |||
MACF1 Antibody (A-3): m-IgGκ BP-HRP Bundle | sc-523359 | 200 µg Ab, 40 µg BP | $361.00 | |||
MACF1 Antibody (A-3): m-IgG1 BP-HRP Bundle | sc-543831 | 200 µg Ab; 20 µg BP | $361.00 | |||
MACF1 Antibody (A-3) AC | sc-377532 AC | 500 µg/ml, 25% agarose | $424.00 | |||
MACF1 Antibody (A-3) HRP | sc-377532 HRP | 200 µg/ml | $322.00 | |||
MACF1 Antibody (A-3) FITC | sc-377532 FITC | 200 µg/ml | $336.00 | |||
MACF1 Antibody (A-3) PE | sc-377532 PE | 200 µg/ml | $349.00 | |||
MACF1 Antibody (A-3) Alexa Fluor® 488 | sc-377532 AF488 | 200 µg/ml | $364.00 | |||
MACF1 Antibody (A-3) Alexa Fluor® 546 | sc-377532 AF546 | 200 µg/ml | $364.00 | |||
MACF1 Antibody (A-3) Alexa Fluor® 594 | sc-377532 AF594 | 200 µg/ml | $364.00 | |||
MACF1 Antibody (A-3) Alexa Fluor® 647 | sc-377532 AF647 | 200 µg/ml | $364.00 | |||
MACF1 Antibody (A-3) Alexa Fluor® 680 | sc-377532 AF680 | 200 µg/ml | $364.00 | |||
MACF1 Antibody (A-3) Alexa Fluor® 790 | sc-377532 AF790 | 200 µg/ml | $364.00 |