M-RIP Antibody (E-1): sc-515720
- M-RIP Antibody (E-1) is a mouse monoclonal IgG1 κ M-RIP antibody provided at 200 µg/ml
- raised against amino acids 668-760 mapping within an internal region of M-RIP of human origin
- M-RIP Antibody (E-1) is recommended for detection of M-RIP of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-M-RIP Antibody (E-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for M-RIP Antibody (E-1) for WB applications. These reagents are now offered in bundles with M-RIP Antibody (E-1) (see ordering information below).
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M-RIP Antibody (E-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects M-RIP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). M-RIP (E-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. M-RIP protein, also known as myosin phosphatase Rho interacting protein, MPRIP, p116Rip, RIP3, or RHOIP3, is a crucial 1,025 amino acid cytoplasmic and cytoskeletal protein that plays a significant role in regulating the actin cytoskeleton. M-RIP′s ability to colocalize with the myosin binding subunit (MBS) is essential for myosin light chain phosphorylation, which is vital for muscle contraction and cellular movement. Additionally, M-RIP interacts with RhoA at actin stress fibers through coiled coil domains, highlighting M-RIP′s importance in cytoskeletal dynamics. M-RIP is predominantly expressed in the ovary, with moderate expression levels in the brain, heart, liver, lung, skeletal muscle, testis, and kidney. Notably, M-RIP depletion leads to increased stress fibers in smooth muscle cells, while M-RIP overexpression results in stress fiber disassembly in neuronal cells, indicating M-RIP′s regulatory role in maintaining cytoskeletal integrity. M-RIP contains two pleckstrin homology (PH) domains and features multiple phosphorylated serine and threonine residues, existing as three isoforms produced by alternative splicing events, which may contribute to M-RIP′s diverse functional roles in cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
M-RIP Antibody (E-1) References:
- p116Rip is a novel filamentous actin-binding protein. | Mulder, J., et al. 2003. J Biol Chem. 278: 27216-23. PMID: 12732640
- Myosin phosphatase-Rho interacting protein. A new member of the myosin phosphatase complex that directly binds RhoA. | Surks, HK., et al. 2003. J Biol Chem. 278: 51484-93. PMID: 14506264
- p116Rip targets myosin phosphatase to the actin cytoskeleton and is essential for RhoA/ROCK-regulated neuritogenesis. | Mulder, J., et al. 2004. Mol Biol Cell. 15: 5516-27. PMID: 15469989
- p116Rip decreases myosin II phosphorylation by activating myosin light chain phosphatase and by inactivating RhoA. | Koga, Y. and Ikebe, M. 2005. J Biol Chem. 280: 4983-91. PMID: 15545284
- M-RIP targets myosin phosphatase to stress fibers to regulate myosin light chain phosphorylation in vascular smooth muscle cells. | Surks, HK., et al. 2005. J Biol Chem. 280: 42543-51. PMID: 16257966
- M-RIP, a novel target of JNK signaling and a requirement for human cancer cell invasion. | Ono, R., et al. 2008. Int J Mol Med. 22: 199-203. PMID: 18636174
- Identification of a novel, putative Rho-specific GDP/GTP exchange factor and a RhoA-binding protein: control of neuronal morphology. | Gebbink, MF., et al. 1997. J Cell Biol. 137: 1603-13. PMID: 9199174
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
M-RIP Antibody (E-1) | sc-515720 | 200 µg/ml | $322.00 | |||
M-RIP Antibody (E-1): m-IgG Fc BP-HRP Bundle | sc-531482 | 200 µg Ab; 10 µg BP | $361.00 | |||
M-RIP Antibody (E-1): m-IgGκ BP-HRP Bundle | sc-525179 | 200 µg Ab, 40 µg BP | $361.00 | |||
M-RIP Antibody (E-1): m-IgG1 BP-HRP Bundle | sc-544581 | 200 µg Ab; 20 µg BP | $361.00 | |||
M-RIP Antibody (E-1) AC | sc-515720 AC | 500 µg/ml, 25% agarose | $424.00 | |||
M-RIP Antibody (E-1) HRP | sc-515720 HRP | 200 µg/ml | $322.00 | |||
M-RIP Antibody (E-1) FITC | sc-515720 FITC | 200 µg/ml | $336.00 | |||
M-RIP Antibody (E-1) PE | sc-515720 PE | 200 µg/ml | $349.00 | |||
M-RIP Antibody (E-1) Alexa Fluor® 488 | sc-515720 AF488 | 200 µg/ml | $364.00 | |||
M-RIP Antibody (E-1) Alexa Fluor® 546 | sc-515720 AF546 | 200 µg/ml | $364.00 | |||
M-RIP Antibody (E-1) Alexa Fluor® 594 | sc-515720 AF594 | 200 µg/ml | $364.00 | |||
M-RIP Antibody (E-1) Alexa Fluor® 647 | sc-515720 AF647 | 200 µg/ml | $364.00 | |||
M-RIP Antibody (E-1) Alexa Fluor® 680 | sc-515720 AF680 | 200 µg/ml | $364.00 | |||
M-RIP Antibody (E-1) Alexa Fluor® 790 | sc-515720 AF790 | 200 µg/ml | $364.00 |