Lunatic Fringe Antibody (TT-07): sc-100756

Lunatic Fringe Antibody (TT-07) is a mouse monoclonal IgG2a kappa light chain antibody that detects Lunatic Fringe protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-Lunatic Fringe antibody (TT-07) is available as the non-conjugated form. Lunatic Fringe protein, also known as LFNG or SCDO3, is a 379 amino acid single-pass type II membrane protein primarily localized to the membrane of the Golgi apparatus. This localization enables Lunatic Fringe to function effectively as a glycosyltransferase, playing a vital role in somite patterning and segmentation during embryonic development. Specifically, Lunatic Fringe initiates the elongation of O-linked fucose residues on Notch molecules, which is essential for proper signaling in the Notch pathway. Disruptions in the gene encoding Lunatic Fringe can lead to autosomal recessive spondylocostal dysostosis 3 (SCDO3), a developmental disorder characterized by rib anomalies and vertebral segmentation issues. Additionally, alternative splicing of the Lunatic Fringe gene results in the expression of three distinct isoforms, further highlighting the complexity and importance of this protein in developmental biology.

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Lunatic Fringe Antibody (TT-07) References:

1. Fringe modifies O-fucose on mouse Notch1 at epidermal growth factor-like repeats within the ligand-binding site and the Abruptex region.  |  Shao, L., et al. 2003. J Biol Chem. 278: 7775-82. PMID: 12486116
2. Mutation of the LUNATIC FRINGE gene in humans causes spondylocostal dysostosis with a severe vertebral phenotype.  |  Sparrow, DB., et al. 2006. Am J Hum Genet. 78: 28-37. PMID: 16385447
3. Expression of Radical fringe in limb-bud ectoderm regulates apical ectodermal ridge formation.  |  Laufer, E., et al. 1997. Nature. 386: 366-73. PMID: 9121552
4. A family of mammalian Fringe genes implicated in boundary determination and the Notch pathway.  |  Johnston, SH., et al. 1997. Development. 124: 2245-54. PMID: 9187150
5. EWS/FLI1-induced manic fringe renders NIH 3T3 cells tumorigenic.  |  May, WA., et al. 1997. Nat Genet. 17: 495-7. PMID: 9398859
6. lunatic fringe is an essential mediator of somite segmentation and patterning.  |  Evrard, YA., et al. 1998. Nature. 394: 377-81. PMID: 9690473
7. The lunatic fringe gene is a target of the molecular clock linked to somite segmentation in avian embryos.  |  McGrew, MJ., et al. 1998. Curr Biol. 8: 979-82. PMID: 9742402
8. Differential expression pattern of the three Fringe genes is associated with epidermal differentiation.  |  Thélu, J., et al. 1998. J Invest Dermatol. 111: 903-6. PMID: 9804358

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Lunatic Fringe Antibody (TT-07) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Lunatic Fringe Antibody (TT-07) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Lunatic Fringe Antibody (TT-07) Specifications

Alternate Product Name: Lunatic Fringe

Product Clone Name: TT-07

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-251

Epitope Target: Lunatic Fringe

Epitope Species: human

Product Reactivity/Detection: Lunatic Fringe

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Lunatic Fringe Antibody (TT-07): N/A