Luciferase Antibody (C-12): sc-74548

Luciferase Antibody (C-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects Luciferase protein of Photinus pyralis origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Luciferase antibody (C-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Luciferase protein, derived from the common North American firefly, Photinus pyralis, is a critical enzyme in bioluminescence, facilitating the conversion of chemical energy into light through a reaction that produces green light at a wavelength of 562 nm. This enzyme is not only pivotal for natural light-emitting processes in organisms but also serves as a powerful reporter in molecular and cellular biology, allowing researchers to visualize and quantify biological events in real-time. The catalytic reaction involves the substrate Photinus luciferin, oxygen, and ATP, resulting in the production of oxidized Photinus luciferin, carbon dioxide, adenosine monophosphate, diphosphate, and light. The ability of Luciferase to emit light in response to specific substrates makes Luciferase an invaluable tool in various applications, including gene expression studies, drug discovery, and in vivo imaging, thereby enhancing our understanding of biological processes and disease mechanisms.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Luciferase Antibody (C-12) References:

1. Concurrent measurement of promoter activity and transfection efficiency using a new reporter vector containing both Photinus pyralis and Renilla reniformis luciferase genes.  |  Park, JB. 2001. Anal Biochem. 291: 162-6. PMID: 11262171
2. Fire fly luciferin as antioxidant and light emitter: the evolution of insect bioluminescence.  |  Dubuisson, M., et al. 2004. Luminescence. 19: 339-44. PMID: 15558801
3. A quantitative high-throughput chemotaxis assay using bioluminescent reporter cells.  |  Vishwanath, RP., et al. 2005. J Immunol Methods. 302: 78-89. PMID: 15987642
4. Red- and green-emitting firefly luciferase mutants for bioluminescent reporter applications.  |  Branchini, BR., et al. 2005. Anal Biochem. 345: 140-8. PMID: 16125663
5. Emission spectra of bioluminescent reporters and interaction with mammalian tissue determine the sensitivity of detection in vivo.  |  Zhao, H., et al. 2005. J Biomed Opt. 10: 41210. PMID: 16178634
6. An enhanced chimeric firefly luciferase-inspired enzyme for ATP detection and bioluminescence reporter and imaging applications.  |  Branchini, BR., et al. 2015. Anal Biochem. 484: 148-53. PMID: 26049097
7. Cloning of the Orange Light-Producing Luciferase from Photinus scintillans-A New Proposal on how Bioluminescence Color is Determined.  |  Branchini, BR., et al. 2017. Photochem Photobiol. 93: 479-485. PMID: 27861940
8. Influence of the C-terminal domain on the bioluminescence activity and color determination in green and red emitting beetle luciferases and luciferase-like enzyme.  |  Bevilaqua, VR., et al. 2021. Photochem Photobiol Sci. 20: 113-122. PMID: 33721241
9. Firefly luciferase is targeted to peroxisomes in mammalian cells.  |  Keller, GA., et al. 1987. Proc Natl Acad Sci U S A. 84: 3264-8. PMID: 3554235
10. Firefly luciferase gene: structure and expression in mammalian cells.  |  de Wet, JR., et al. 1987. Mol Cell Biol. 7: 725-37. PMID: 3821727
11. Structural basis for the inhibition of firefly luciferase by a general anesthetic.  |  Franks, NP., et al. 1998. Biophys J. 75: 2205-11. PMID: 9788915
12. Synthesis of active firefly luciferase by in vitro translation of RNA obtained from adult lanterns.  |  Wood, KV., et al. 1984. Biochem Biophys Res Commun. 124: 592-6. PMID: 6208909

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Luciferase Antibody (C-12) has 29 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Luciferase Antibody (C-12) Specifications

Alternate Product Name: Luciferase

Product Clone Name: C-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 251-550

Epitope Target: Luciferase

Epitope Species: Photinus pyralis

Product Reactivity/Detection: Luciferase

Species Reactivity/Detection: Photinus pyralis

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 29 citations in various scientific publications

Available Conjugates/Forms for Luciferase Antibody (C-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin