Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
LTBP-4 CRISPR/Cas9 KO Plasmid (h): sc-405419
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LTBP-4 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the LTBP-4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LTBP-4 Antibody (A-2): sc-393666
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LTBP-4 CRISPR/Cas9 KO Plasmid (h) | sc-405419 | 20 µg | $397.00 |
Overview
LTBP4 encodes latent transforming growth factor beta binding protein 4 (LTBP-4), an extracellular matrix glycoprotein that associates with fibrillin microfibrils and regulates sequestration, storage, and bioavailability of latent TGF-β complexes. Through these interactions, LTBP-4 influences TGF-β pathway signaling dynamics and contributes to matrix assembly and remodeling programs that shape cell adhesion, mechanotransduction, and tissue elasticity. LTBP4 function is closely linked to elastic fiber homeostasis via coordination with elastin and microfibrillar components, affecting structural integrity in connective tissues. Genetic disruption or altered expression of LTBP4 has been implicated in connective tissue and cardiopulmonary phenotypes, supporting its relevance for studies of extracellular matrix biology and TGF-β–dependent disease mechanisms.
LTBP-4 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the LTBP4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the LTBP4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the LTBP4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish LTBP-4 protein expression.
This CRISPR knockout system enables efficient generation of LTBP4-deficient cell models for investigation of LTBP-4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting LTBP4 exon(s) critical for LTBP-4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple LTBP4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by LTBP-4 CRISPR/Cas9 KO Plasmid (h) and LTBP-4 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the LTBP4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by LTBP-4 HDR Plasmid (h) and LTBP-4 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by LTBP4 homology arms to support homology-directed repair at defined LTBP4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.