LSm8 Antibody (F-8): sc-390542

LSm8 Antibody (F-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects LSm8 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). LSm8 (F-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. LSm8, also known as YJR022W, is a crucial component of the LSm2-8 complex, which is essential for processing small nucleolar RNAs (snoRNAs), transfer RNAs (tRNAs), and ribosomal RNAs (rRNAs), as well as turnover of precursor messenger RNAs (pre-mRNAs). LSm2-8 complex plays a vital role in RNA metabolism, facilitating RNA-protein interactions and structural changes necessary for ribosomal subunit assembly. LSm8 is predominantly localized in the nucleus, where LSm8 specifically binds to the 3′-terminal U-tract of U6 small nuclear RNA (snRNA), ensuring proper nuclear localization of U6 snRNA, which is critical for spliceosome assembly and function. LSm8′s ability to form heptameric complexes underscores LSm8′s importance in maintaining RNA stability and regulating gene expression, making LSm8 a significant target for research in molecular biology and genetics.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

LSm8 Antibody (F-8) References:

1. Multiple functional interactions between components of the Lsm2-Lsm8 complex, U6 snRNA, and the yeast La protein.  |  Pannone, BK., et al. 2001. Genetics. 158: 187-96. PMID: 11333229
2. Xenopus LSm proteins bind U8 snoRNA via an internal evolutionarily conserved octamer sequence.  |  Tomasevic, N. and Peculis, BA. 2002. Mol Cell Biol. 22: 4101-12. PMID: 12024024
3. Lsm Proteins are required for normal processing and stability of ribosomal RNAs.  |  Kufel, J., et al. 2003. J Biol Chem. 278: 2147-56. PMID: 12438310
4. The human LSm1-7 proteins colocalize with the mRNA-degrading enzymes Dcp1/2 and Xrnl in distinct cytoplasmic foci.  |  Ingelfinger, D., et al. 2002. RNA. 8: 1489-501. PMID: 12515382
5. An Lsm2-Lsm7 complex in Saccharomyces cerevisiae associates with the small nucleolar RNA snR5.  |  Fernandez, CF., et al. 2004. Mol Biol Cell. 15: 2842-52. PMID: 15075370
6. Nuclear pre-mRNA decapping and 5' degradation in yeast require the Lsm2-8p complex.  |  Kufel, J., et al. 2004. Mol Cell Biol. 24: 9646-57. PMID: 15485930
7. The Lsm2-8 complex determines nuclear localization of the spliceosomal U6 snRNA.  |  Spiller, MP., et al. 2007. Nucleic Acids Res. 35: 923-9. PMID: 17251193
8. A role for the yeast La protein in U6 snRNP assembly: evidence that the La protein is a molecular chaperone for RNA polymerase III transcripts.  |  Pannone, BK., et al. 1998. EMBO J. 17: 7442-53. PMID: 9857199

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-LSm8 Antibody (F-8) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-LSm8 Antibody (F-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

LSm8 Antibody (F-8) Specifications

Alternate Product Name: LSm8

Product Clone Name: F-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-96

Epitope Target: LSm8

Epitope Species: human

Product Reactivity/Detection: LSm8

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for LSm8 Antibody (F-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent