Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
LSDP5 CRISPR/Cas9 KO Plasmid (m): sc-426297
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LSDP5 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the LSDP5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LSDP5 Antibody (E-3): sc-514296
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LSDP5 CRISPR/Cas9 KO Plasmid (m) | sc-426297 | 20 µg | $397.00 |
Overview
Perilipin 5 (Plin5), also known as lipid storage droplet protein 5 (LSDP5), is a lipid droplet–associated protein that regulates intracellular triacylglycerol storage and coordinates fatty acid trafficking to mitochondria for β-oxidation. In mouse oxidative tissues such as heart, liver, and skeletal muscle, PLIN5 helps balance lipid sequestration with lipolysis by modulating access of lipases to the droplet surface and coupling lipid mobilization to mitochondrial oxidative capacity. Through these functions, PLIN5 influences energy homeostasis, lipid-induced signaling, and cellular responses to nutrient availability and fasting. Dysregulation of PLIN5-linked lipid droplet dynamics is relevant to experimental models of steatosis, insulin resistance, and lipotoxic stress that impact mitochondrial function and inflammation-associated metabolic phenotypes.
LSDP5 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Plin5 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Plin5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Plin5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish LSDP5 protein expression.
This CRISPR knockout system enables efficient generation of Plin5-deficient cell models for investigation of LSDP5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Plin5 exon(s) critical for LSDP5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Plin5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by LSDP5 CRISPR/Cas9 KO Plasmid (m) and LSDP5 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Plin5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by LSDP5 HDR Plasmid (m) and LSDP5 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Plin5 homology arms to support homology-directed repair at defined Plin5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.