LSD1 Antibody (1B2E5): sc-53875
- LSD1 Antibody (1B2E5) is a mouse monoclonal IgG1 κ, cited in 6 publications, provided at 200 µg/ml
- raised against purified truncated recombinant LSD1 of human origin
- recommended for detection of LSD1 of mouse, rat and human origin by WB, IP and IF
- m-IgGκ BP-HRP is the preferred secondary detection reagent for LSD1 Antibody (1B2E5) for WB applications. This reagent is now offered in a bundle with LSD1 Antibody (1B2E5) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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LSD1 Antibody (1B2E5) is a mouse monoclonal IgG1 antibody that detects LSD1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). LSD1, also known as lysine-specific histone demethylase 1 or BHC110, plays a crucial role in the regulation of chromatin structure and gene expression by removing methyl groups from histone H3, specifically from the methyl-lysine-4 side chain. This demethylation process is vital for maintaining an epigenetic state within the cell, influencing cellular differentiation and development. LSD1′s structure includes a SWIRM domain, a flavin adenine dinucleotide (FAD)-binding motif, and an amine oxidase domain, which are essential for enzymatic activity. Additionally, LSD1 interacts with CoREST, a corepressor that enhances demethylation capabilities and protects against degradation, thereby ensuring stability and function in the cell. LSD1 regulation is further complicated by the presence of PHF21A, a PHD domain-containing protein that inhibits activity, highlighting the intricate balance of protein interactions that govern histone methylation dynamics. LSD1′s co-localization with the androgen receptor in prostate cells underscores significance in androgen-receptor-dependent transcription, making LSD1 a critical player in both normal physiology and disease states, such as cancer.
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LSD1 Antibody (1B2E5) References:
- Histone demethylation mediated by the nuclear amine oxidase homolog LSD1. | Shi, Y., et al. 2004. Cell. 119: 941-53. PMID: 15620353
- LSD1 Ablation Stimulates Anti-tumor Immunity and Enables Checkpoint Blockade. | Sheng, W., et al. 2018. Cell. 174: 549-563.e19. PMID: 29937226
- Re-programing Chromatin with a Bifunctional LSD1/HDAC Inhibitor Induces Therapeutic Differentiation in DIPG. | Anastas, JN., et al. 2019. Cancer Cell. 36: 528-544.e10. PMID: 31631026
- LSD1/KDM1A inhibitors in clinical trials: advances and prospects. | Fang, Y., et al. 2019. J Hematol Oncol. 12: 129. PMID: 31801559
- Myofibroblast Deficiency of LSD1 Alleviates TAC-Induced Heart Failure. | Huo, JL., et al. 2021. Circ Res. 129: 400-413. PMID: 34078090
- LSD1-Demethylated LINC01134 Confers Oxaliplatin Resistance Through SP1-Induced p62 Transcription in HCC. | Ma, L., et al. 2021. Hepatology. 74: 3213-3234. PMID: 34322883
- Demethylase-independent roles of LSD1 in regulating enhancers and cell fate transition. | Zeng, C., et al. 2023. Nat Commun. 14: 4944. PMID: 37607921
- Lysine-Specific Demethylase 1 (LSD1) Inhibitors: Peptides as an Emerging Class of Therapeutics. | Baby, S., et al. 2023. ACS Chem Biol. 18: 2144-2155. PMID: 37812385
- Caloric restriction leads to druggable LSD1-dependent cancer stem cells expansion. | Pallavi, R., et al. 2024. Nat Commun. 15: 828. PMID: 38280853
- The scaffolding function of LSD1 controls DNA methylation in mouse ESCs. | Malla, S., et al. 2024. Nat Commun. 15: 7758. PMID: 39237615
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LSD1 Antibody (1B2E5) | sc-53875 | 200 µg/ml | $322.00 | |||
LSD1 Antibody (1B2E5): m-IgGκ BP-HRP Bundle | sc-520995 | 200 µg Ab, 40 µg BP | $361.00 |