LRFN4 Antibody (F-3): sc-393425

LRFN4 Antibody (F-3) is a mouse monoclonal IgG3 kappa light chain antibody that detects LRFN4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-LRFN4 antibody (F-3) is available as the non-conjugated form. LRFN4, also known as leucine-rich repeat and fibronectin type III domain containing 4, SALM3, or FIGLER6, is a 635 amino acid single-pass type I membrane protein that plays a crucial role in neuronal development and synaptic function. LRFN4 is primarily located in the cell membrane, where LRFN4 promotes neurite outgrowth in hippocampal neurons, essential for neural network formation and proper brain function. The presence of a fibronectin type-III domain, an immunoglobulin-like domain, and multiple leucine-rich repeats in LRFN4′s structure allows interactions with various proteins, including PSD-95, a key scaffolding protein in synapses. These interactions are vital for PSD-95 redistribution to the cell periphery, thereby influencing synaptic plasticity and stability. LRFN4 forms heteromeric complexes with other members of the LRFN family, such as LRFN1, LRFN2, LRFN3, and LRFN5, but LRFN4 does not form homomeric complexes across cell junctions like some other family members. LRFN4′s PDZ-binding motif is critical for promoting neurite outgrowth and binding to scaffolding proteins like SAP 97 and NE-dlg. The gene encoding LRFN4 is located on human chromosome 11q13.1 and mouse chromosome 19 A, highlighting evolutionary conservation and importance across species.

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LRFN4 Antibody (F-3) References:

1. A novel family of adhesion-like molecules that interacts with the NMDA receptor.  |  Wang, CY., et al. 2006. J Neurosci. 26: 2174-83. PMID: 16495444
2. SALM synaptic cell adhesion-like molecules regulate the differentiation of excitatory synapses.  |  Ko, J., et al. 2006. Neuron. 50: 233-45. PMID: 16630835
3. Comparative analysis of structure, expression and PSD95-binding capacity of Lrfn, a novel family of neuronal transmembrane proteins.  |  Morimura, N., et al. 2006. Gene. 380: 72-83. PMID: 16828986
4. Leucine-rich repeat proteins of synapses.  |  Ko, J. and Kim, E. 2007. J Neurosci Res. 85: 2824-32. PMID: 17471552
5. Regulation of erythropoiesis by the neuronal transmembrane protein Lrfn2.  |  Castellanos, A., et al. 2007. Exp Hematol. 35: 724-34. PMID: 17577922
6. The SALM family of adhesion-like molecules forms heteromeric and homomeric complexes.  |  Seabold, GK., et al. 2008. J Biol Chem. 283: 8395-405. PMID: 18227064
7. Synaptic adhesion-like molecules (SALMs) promote neurite outgrowth.  |  Wang, PY., et al. 2008. Mol Cell Neurosci. 39: 83-94. PMID: 18585462
8. LRF is an essential downstream target of GATA1 in erythroid development and regulates BIM-dependent apoptosis.  |  Maeda, T., et al. 2009. Dev Cell. 17: 527-40. PMID: 19853566
9. Selected SALM (synaptic adhesion-like molecule) family proteins regulate synapse formation.  |  Mah, W., et al. 2010. J Neurosci. 30: 5559-68. PMID: 20410109
10. The LRF transcription factor regulates mature B cell development and the germinal center response in mice.  |  Sakurai, N., et al. 2011. J Clin Invest. 121: 2583-98. PMID: 21646720
11. LRF-mediated Dll4 repression in erythroblasts is necessary for hematopoietic stem cell maintenance.  |  Lee, SU., et al. 2013. Blood. 121: 918-29. PMID: 23134786
12. LRF inhibits p53 expression in colon cancer cells via modulating DAP5 activity.  |  Zhu, M., et al. 2017. Cell Biochem Funct. 35: 401-406. PMID: 28849590

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-LRFN4 Antibody (F-3) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-LRFN4 Antibody (F-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

LRFN4 Antibody (F-3) Specifications

Alternate Product Name: LRFN4

Product Clone Name: F-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 209-232

Epitope Target: LRFN4

Epitope Species: human

Product Reactivity/Detection: LRFN4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for LRFN4 Antibody (F-3): P: Blocking Peptide