LPCAT2 Antibody (H-7): sc-514354
- LPCAT2 Antibody (H-7) is a mouse monoclonal IgG1 κ LPCAT2 antibody provided at 200 µg/ml
- raised against amino acids 376-449 mapping near the C-terminus of LPCAT2 of human origin
- LPCAT2 Antibody (H-7) is recommended for detection of LPCAT2 of human origin by WB, IP, IF and ELISA
- Anti-LPCAT2 Antibody (H-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for LPCAT2 Antibody (H-7) for WB applications. These reagents are now offered in bundles with LPCAT2 Antibody (H-7) (see ordering information below).
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LPCAT2 Antibody (H-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects LPCAT2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). LPCAT2 (H-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. LPCAT2, or lysophosphatidylcholine acyltransferase 2, is a 544 amino acid protein that belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family and is primarily located in the endoplasmic reticulum and Golgi apparatus membranes. This localization is crucial as LPCAT2 effectively participates in lipid and phospholipid metabolism, particularly in the biosynthesis of platelet-activating factor (PAF). LPCAT2 exhibits both acyltransferase and acetyltransferase activities, which are calcium-dependent, allowing LPCAT2 to catalyze the conversion of the PAF precursor, lyso-PAF, into PAF, as well as converting lyso-PAF into 1-alkyl-phosphatidylcholine, a key component of cell membranes. During resting states, LPCAT2′s acyltransferase activity predominates, while acute inflammatory stimuli, such as lipopolysaccharides, enhance acetyltransferase activity, leading to increased PAF synthesis. This dynamic regulation underscores LPCAT2′s vital role in cellular responses to inflammation and membrane composition.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
LPCAT2 Antibody (H-7) References:
- A single enzyme catalyzes both platelet-activating factor production and membrane biogenesis of inflammatory cells. Cloning and characterization of acetyl-CoA:LYSO-PAF acetyltransferase. | Shindou, H., et al. 2007. J Biol Chem. 282: 6532-9. PMID: 17182612
- Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip. | Talmud, PJ., et al. 2009. Am J Hum Genet. 85: 628-42. PMID: 19913121
- Enzymatic activity of the human 1-acylglycerol-3-phosphate-O-acyltransferase isoform 11: upregulated in breast and cervical cancers. | Agarwal, AK. and Garg, A. 2010. J Lipid Res. 51: 2143-52. PMID: 20363836
- Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study. | Bailey, SD., et al. 2010. Diabetes Care. 33: 2250-3. PMID: 20628086
- Single nucleotide polymorphisms of matrix metalloproteinase 9 (MMP9) and tumor protein 73 (TP73) interact with Epstein-Barr virus in chronic lymphocytic leukemia: results from the European case-control study EpiLymph. | Casabonne, D., et al. 2011. Haematologica. 96: 323-7. PMID: 21048031
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LPCAT2 Antibody (H-7) | sc-514354 | 200 µg/ml | $322.00 | |||
LPCAT2 Antibody (H-7): m-IgG Fc BP-HRP Bundle | sc-531097 | 200 µg Ab; 10 µg BP | $361.00 | |||
LPCAT2 Antibody (H-7): m-IgGκ BP-HRP Bundle | sc-524694 | 200 µg Ab, 40 µg BP | $361.00 | |||
LPCAT2 Antibody (H-7): m-IgG1 BP-HRP Bundle | sc-544395 | 200 µg Ab; 20 µg BP | $361.00 | |||
LPCAT2 Antibody (H-7) AC | sc-514354 AC | 500 µg/ml, 25% agarose | $424.00 | |||
LPCAT2 Antibody (H-7) HRP | sc-514354 HRP | 200 µg/ml | $322.00 | |||
LPCAT2 Antibody (H-7) FITC | sc-514354 FITC | 200 µg/ml | $336.00 | |||
LPCAT2 Antibody (H-7) PE | sc-514354 PE | 200 µg/ml | $349.00 | |||
LPCAT2 Antibody (H-7) Alexa Fluor® 488 | sc-514354 AF488 | 200 µg/ml | $364.00 | |||
LPCAT2 Antibody (H-7) Alexa Fluor® 546 | sc-514354 AF546 | 200 µg/ml | $364.00 | |||
LPCAT2 Antibody (H-7) Alexa Fluor® 594 | sc-514354 AF594 | 200 µg/ml | $364.00 | |||
LPCAT2 Antibody (H-7) Alexa Fluor® 647 | sc-514354 AF647 | 200 µg/ml | $364.00 | |||
LPCAT2 Antibody (H-7) Alexa Fluor® 680 | sc-514354 AF680 | 200 µg/ml | $364.00 | |||
LPCAT2 Antibody (H-7) Alexa Fluor® 790 | sc-514354 AF790 | 200 µg/ml | $364.00 |