LOK CRISPR/Cas9 KO Plasmid (m): sc-423191
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LOK CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the LOK genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LOK Antibody (D-6): sc-398083
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LOK CRISPR/Cas9 KO Plasmid (m) | sc-423191 | 20 µg | $397.00 |
Overview
Stk10 encodes lymphocyte-oriented kinase (LOK), a STE20 family serine/threonine kinase that links upstream signaling to cytoskeletal remodeling and immune cell behavior in mouse. LOK participates in pathways governing actin dynamics, cell polarity, and adhesion, influencing processes such as lymphocyte migration, immunological synapse organization, and responsiveness to activation cues. In hematopoietic contexts, altered STK10/LOK signaling has been associated with dysregulated immune homeostasis and aberrant cell motility phenotypes, making it relevant for studies of inflammation-linked biology. LOK function also intersects with kinase-controlled checkpoints that shape proliferation and survival programs, supporting mechanistic work on signaling networks in normal and disease-relevant cell states.
LOK CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Stk10 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Stk10 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Stk10 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish LOK protein expression.
This CRISPR knockout system enables efficient generation of Stk10-deficient cell models for investigation of LOK signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Stk10 exon(s) critical for LOK function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Stk10 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by LOK CRISPR/Cas9 KO Plasmid (m) and LOK CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Stk10 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by LOK HDR Plasmid (m) and LOK HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Stk10 homology arms to support homology-directed repair at defined Stk10 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.