Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
LOK CRISPR/Cas9 KO Plasmid (h): sc-405859
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LOK CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the LOK genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LOK Antibody (D-6): sc-398083
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LOK CRISPR/Cas9 KO Plasmid (h) | sc-405859 | 20 µg | $397.00 |
Overview
Serine/threonine-protein kinase 10 (STK10), also known as lymphocyte-oriented kinase (LOK), is a STE20-family kinase that regulates cytoskeletal organization and cell polarity through phosphorylation events that influence actin dynamics and ERM (ezrin–radixin–moesin) protein function. In immune cells, LOK contributes to lymphocyte adhesion, migration, and immunological synapse architecture, linking receptor signaling to cortical actin remodeling. STK10 activity interfaces with Rho-family GTPase–dependent pathways and broader signaling networks that coordinate motility and tissue trafficking. Dysregulated cytoskeletal signaling and aberrant kinase-mediated control of cell movement have been associated with immune dysfunction and cancer-related phenotypes, making STK10 a relevant target for mechanistic studies.
LOK CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the STK10 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the STK10 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the STK10 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish LOK protein expression.
This CRISPR knockout system enables efficient generation of STK10-deficient cell models for investigation of LOK signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting STK10 exon(s) critical for LOK function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple STK10 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by LOK CRISPR/Cas9 KO Plasmid (h) and LOK CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the STK10 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by LOK HDR Plasmid (h) and LOK HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by STK10 homology arms to support homology-directed repair at defined STK10 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.