Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
LMTK3 CRISPR/Cas9 KO Plasmid (h): sc-403191
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LMTK3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the LMTK3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LMTK3 Antibody (I-17): sc-100418
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LMTK3 CRISPR/Cas9 KO Plasmid (h) | sc-403191 | 20 µg | $397.00 |
Overview
LMTK3 (lemur tyrosine kinase 3) encodes a membrane-associated serine/threonine kinase implicated in regulating intracellular signaling and vesicle trafficking pathways that influence receptor turnover and signal duration. In epithelial contexts, LMTK3 has been linked to modulation of kinase-driven transcriptional programs and crosstalk with hormone receptor signaling, shaping cellular proliferation and stress responses. Altered LMTK3 expression or activity has been reported in multiple cancer-relevant settings, where it is studied for its impact on growth-factor responsiveness, survival signaling, and therapy-associated resistance phenotypes. As a result, LMTK3 is a useful target for dissecting kinase network wiring, endomembrane dynamics, and downstream transcriptional outputs in human model systems.
LMTK3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the LMTK3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the LMTK3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the LMTK3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish LMTK3 protein expression.
This CRISPR knockout system enables efficient generation of LMTK3-deficient cell models for investigation of LMTK3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting LMTK3 exon(s) critical for LMTK3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple LMTK3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by LMTK3 CRISPR/Cas9 KO Plasmid (h) and LMTK3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the LMTK3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by LMTK3 HDR Plasmid (h) and LMTK3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by LMTK3 homology arms to support homology-directed repair at defined LMTK3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.