LMTK3 Antibody (I-17) recommended for detection of LMTK3 of mouse, rat and human origin by WB, IP and ELISA

LMTK3 Antibody (I-17): sc-100418

LMTK3 Antibody (I-17) is rated 3.3 out of 5 by 3.
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Datasheets

    • Anti-LMTK3 Antibody (I-17) is a mouse monoclonal IgG1 (kappa light chain) LMTK3 antibody provided at 50 µg/0.5 ml
    • raised against a partial recombinant protein mapping within amino acids 1151-1250 of LMTK3 of human origin
    • recommended for detection of LMTK3 of mouse, rat and human origin by WB, IP and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of LMTK3 (I-17): sc-100418.

What is the recommended starting dilution for Western blot for LMTK3 (I-17): sc-100418 monoclonal antibodyy?

Asked by: TinTin
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Tech Service
Date published: 2017-02-02
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Rated 1 out of 5 by from Erster Blot leider erfolglos Prima war, daß ich innerhalb 1 Woche eine kostenlose Probe des LMTK3 (I-17) Antikörpers zugeschickt bekam. Meine erster Blot blieb leider ohne Signal/nur Unspezifisches. Hatte 20µg Gesamtprotein geladen und nach unserem Standard-Protokoll geblottet. Werde aber noch nicht aufgeben, ggf. mehr Lysat nötig, bzw. Kernfraktion bzw. Änderung des Blottens nötig, da 154kDa doch schon ein höheres MW ist. Lt. Datasheet zeigt dieser Ab ja auch ein paar unspezifische Banden. Bleibe dran und bedanke mich trotzdem herzlich für die kostenlose Probe!
Date published: 2017-08-02
Rated 5 out of 5 by from Works great in western blot We use this antibody for western blot and we got a great band around the correct molecular weight
Date published: 2017-01-10
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