LMP7 Antibody (A-12): sc-365699

LMP7 Antibody (A-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects LMP7 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). LMP7 (A-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. LMP7 is a crucial component of the proteasome, a multi-catalytic proteinase complex located in both the nucleus and cytoplasm of eukaryotic cells. This complex plays a vital role in the ATP/Ubiquitin-dependent proteolytic pathway, which is essential for maintaining cellular homeostasis by degrading misfolded or damaged proteins. The proteasome′s structure, characterized by a highly ordered ring-shaped assembly of at least 15 nonidentical subunits, allows for multiple distinct proteolytic activities, including the cleavage of peptides after branched-chain or bulky hydrophobic amino acids. LMP7, along with LMP2, links the proteasome with the MHC class I antigen-processing pathway, particularly upon stimulation with interferon-gamma (IFN-γ), which prompts the displacement of housekeeping subunits and activates cytotoxic T cells. LMP7 exists in two forms, LMP7A and LMP7B, across various tissues, highlighting its significance in immune response and cellular regulation.

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LMP7 Antibody (A-12) References:

1. Interferon-gamma inducible exchanges of 20S proteasome active site subunits: why?  |  Groettrup, M., et al. 2001. Biochimie. 83: 367-72. PMID: 11295499
2. Alternative exon usage and processing of the major histocompatibility complex-encoded proteasome subunits.  |  Früh, K., et al. 1992. J Biol Chem. 267: 22131-40. PMID: 1429565
3. Immunoproteasome subunit LMP7 deficiency and inhibition suppresses Th1 and Th17 but enhances regulatory T cell differentiation.  |  Kalim, KW., et al. 2012. J Immunol. 189: 4182-93. PMID: 22984077
4. miR-451 suppresses the NF-kappaB-mediated proinflammatory molecules expression through inhibiting LMP7 in diabetic nephropathy.  |  Sun, Y., et al. 2016. Mol Cell Endocrinol. 433: 75-86. PMID: 27264074
5. Prevention of colitis-associated cancer by selective targeting of immunoproteasome subunit LMP7.  |  Vachharajani, N., et al. 2017. Oncotarget. 8: 50447-50459. PMID: 28881574
6. Genetic variability in LMP2 and LMP7 is associated with the risk of esophageal squamous cell carcinoma in the Kazakh population but is not associated with HPV infection.  |  Yang, L., et al. 2017. PLoS One. 12: e0186319. PMID: 29073155
7. Immunohistochemical Identification and Assessment of the Location of Immunoproteasome Subunits LMP2 and LMP7 in Acquired Cholesteatoma.  |  Rutkowska, J., et al. 2023. Int J Mol Sci. 24: PMID: 37762439
8. Catalytic components of the bovine pituitary multicatalytic proteinase complex (proteasome).  |  Cardozo, C. 1993. Enzyme Protein. 47: 296-305. PMID: 7697127
9. Major histocompatibility-encoded human proteasome LMP2. Genomic organization and a new form of mRNA.  |  Singal, DP., et al. 1995. J Biol Chem. 270: 1966-70. PMID: 7829535
10. Dissociation and reassociation of the bovine pituitary multicatalytic proteinase complex.  |  Figueiredo-Pereira, ME., et al. 1994. J Biol Chem. 269: 621-6. PMID: 8276861
11. The major-histocompatibility-complex-encoded beta-type proteasome subunits LMP2 and LMP7. Evidence that LMP2 and LMP7 are synthesized as proproteins and that cellular levels of both mRNA and LMP-containing 20S proteasomes are differentially regulated.  |  Frentzel, S., et al. 1993. Eur J Biochem. 216: 119-26. PMID: 8365398
12. The major histocompatibility complex-encoded proteasome component LMP7: alternative first exons and post-translational processing.  |  Glynne, R., et al. 1993. Eur J Immunol. 23: 860-6. PMID: 8458375

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-LMP7 Antibody (A-12) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-LMP7 Antibody (A-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

LMP7 Antibody (A-12) Specifications

Alternate Product Name: LMP7

Product Clone Name: A-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 249-272

Epitope Target: LMP7

Epitope Species: human

Product Reactivity/Detection: LMP7A and LMP7B

Species Reactivity/Detection: human

Additional Mammalian Reactivity: equine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for LMP7 Antibody (A-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin