Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
LLH1 CRISPR/Cas9 KO Plasmid (m): sc-422311
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LLH1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the LLH1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LLH1 Antibody (B-5): sc-271640
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LLH1 CRISPR/Cas9 KO Plasmid (m) | sc-422311 | 20 µg | $397.00 |
Overview
Plod1 encodes lysyl hydroxylase 1 (LLH1), an endoplasmic reticulum enzyme that hydroxylates specific lysine residues in procollagens, enabling subsequent glycosylation and stabilizing collagen cross-linking. Through regulation of collagen maturation and extracellular matrix organization, LLH1 influences connective tissue integrity, basement membrane structure, and tissue remodeling pathways. Perturbation of Plod1 activity is linked to abnormal collagen fibrillogenesis and matrix mechanics, processes relevant to fibrosis biology, vascular and skin fragility phenotypes, and tumor microenvironment studies in mouse models. As a result, Plod1 is commonly investigated in ECM-driven signaling, cell–matrix interactions, and mechanotransduction contexts.
LLH1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Plod1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Plod1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Plod1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish LLH1 protein expression.
This CRISPR knockout system enables efficient generation of Plod1-deficient cell models for investigation of LLH1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Plod1 exon(s) critical for LLH1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Plod1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by LLH1 CRISPR/Cas9 KO Plasmid (m) and LLH1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Plod1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by LLH1 HDR Plasmid (m) and LLH1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Plod1 homology arms to support homology-directed repair at defined Plod1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.