LLH1 Antibody (B-5): sc-271640
- LLH1 Antibody (B-5) is a mouse monoclonal IgG1 κ LLH1 antibody, cited in 4 publications, provided at 200 µg/ml
- raised against amino acids 298-363 mapping within an internal region of LLH1 of human origin
- LLH1 Antibody (B-5) is recommended for detection of LLH1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-LLH1 Antibody (B-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for LLH1 Antibody (B-5) for WB and IHC(P) applications. These reagents are now offered in bundles with LLH1 Antibody (B-5) (see ordering information below).
QUICK LINKS
LLH1 Antibody (B-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects LLH1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). LLH1 (B-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. LLH1 plays a critical role in collagen biosynthesis, specifically by hydroxylating lysine residues within collagen′s triple helix structure, which is essential for the formation of stable cross-links that provide tensile strength to connective tissues. This post-translational modification is vital for maintaining the structural integrity of collagen, and any deficiency in LLH1 can lead to serious connective tissue disorders, such as Ehlers-Danlos syndrome type VI, characterized by joint hypermobility and skin fragility. LLH1 is predominantly expressed in tissues such as the liver, heart, lung, skeletal muscle, and kidney, highlighting its importance in various physiological processes. Anti-LLH1 antibody (B-5) effectively detects this protein across multiple applications, making LLH1 (B-5) monoclonal antibody an invaluable tool for researchers studying collagen-related diseases and the underlying mechanisms of connective tissue disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
LLH1 Antibody (B-5) References:
- Characterization of cDNAs for mouse lysyl hydroxylase 1, 2 and 3, their phylogenetic analysis and tissue-specific expression in the mouse. | Ruotsalainen, H., et al. 1999. Matrix Biol. 18: 325-9. PMID: 10429951
- Deletion of cysteine 369 in lysyl hydroxylase 1 eliminates enzyme activity and causes Ehlers-Danlos syndrome type VI. | Yeowell, HN., et al. 2000. Matrix Biol. 19: 37-46. PMID: 10686424
- Mutations in the lysyl hydroxylase 1 gene that result in enzyme deficiency and the clinical phenotype of Ehlers-Danlos syndrome type VI. | Yeowell, HN. and Walker, LC. 2000. Mol Genet Metab. 71: 212-24. PMID: 11001813
- Characterization of collagenous peptides bound to lysyl hydroxylase isoforms. | Risteli, M., et al. 2004. J Biol Chem. 279: 37535-43. PMID: 15208310
- A novel mutation in the lysyl hydroxylase 1 gene causes decreased lysyl hydroxylase activity in an Ehlers-Danlos VIA patient. | Walker, LC., et al. 2005. J Invest Dermatol. 124: 914-8. PMID: 15854030
- Minoxidil exerts different inhibitory effects on gene expression of lysyl hydroxylase 1, 2, and 3: implications for collagen cross-linking and treatment of fibrosis. | Zuurmond, AM., et al. 2005. Matrix Biol. 24: 261-70. PMID: 15908192
- Differential gene expression of collagen-binding small leucine-rich proteoglycans and lysyl hydroxylases, during mineralization by MC3T3-E1 cells cultured on titanium implant material. | Takashi, M., et al. 2005. Eur J Oral Sci. 113: 225-31. PMID: 15953247
- Identification of arginine-700 as the residue that binds the C-5 carboxyl group of 2-oxoglutarate in human lysyl hydroxylase 1. | Passoja, K., et al. 1998. FEBS Lett. 434: 145-8. PMID: 9738467
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LLH1 Antibody (B-5) | sc-271640 | 200 µg/ml | $322.00 | |||
LLH1 Antibody (B-5): m-IgG Fc BP-HRP Bundle | sc-529228 | 200 µg Ab; 10 µg BP | $361.00 | |||
LLH1 Antibody (B-5): m-IgGκ BP-HRP Bundle | sc-522005 | 200 µg Ab, 40 µg BP | $361.00 | |||
LLH1 Antibody (B-5): m-IgG1 BP-HRP Bundle | sc-543400 | 200 µg Ab; 20 µg BP | $361.00 | |||
LLH1 Antibody (B-5) AC | sc-271640 AC | 500 µg/ml, 25% agarose | $424.00 | |||
LLH1 Antibody (B-5) HRP | sc-271640 HRP | 200 µg/ml | $322.00 | |||
LLH1 Antibody (B-5) FITC | sc-271640 FITC | 200 µg/ml | $336.00 | |||
LLH1 Antibody (B-5) PE | sc-271640 PE | 200 µg/ml | $349.00 | |||
LLH1 Antibody (B-5) Alexa Fluor® 488 | sc-271640 AF488 | 200 µg/ml | $364.00 | |||
LLH1 Antibody (B-5) Alexa Fluor® 546 | sc-271640 AF546 | 200 µg/ml | $364.00 | |||
LLH1 Antibody (B-5) Alexa Fluor® 594 | sc-271640 AF594 | 200 µg/ml | $364.00 | |||
LLH1 Antibody (B-5) Alexa Fluor® 647 | sc-271640 AF647 | 200 µg/ml | $364.00 | |||
LLH1 Antibody (B-5) Alexa Fluor® 680 | sc-271640 AF680 | 200 µg/ml | $364.00 | |||
LLH1 Antibody (B-5) Alexa Fluor® 790 | sc-271640 AF790 | 200 µg/ml | $364.00 |