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LKB1 Antibody (Ley 37D/G6) - Western Blotting - Image 8912
LKB1 抗体 (Ley 37D/G6): sc-32245. A-431 (A), 3611-RF (B) 和 MCF7 (C) 全细胞裂解液中 LKB1 表达的 Western 印迹分析.
LKB1 抗体 (Ley 37D/G6): sc-32245. A-431 (A), 3611-RF (B) 和 MCF7 (C) 全细胞裂解液中 LKB1 表达的 Western 印迹分析.

LKB1 抗体 (Ley 37D/G6): sc-32245

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说明书
  • LKB1 抗体 Ley 37D/G6 是小鼠单克隆 IgG2b κ，LKB1抗体, 在118篇文献中引用，规格为200 µg/ml
  • 免疫human物种的重组LKB1
  • LKB1 抗体 (Ley 37D/G6) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 LKB1
  • 抗LKB1抗体（Ley 37D/G6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取LKB1 (Ley 37D/G6): sc-32245的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 LKB1 抗体 (Ley 37D/G6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与LKB1 抗体 (Ley 37D/G6) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

LKB1抗体（Ley 37D/G6）是一种小鼠单克隆IgG2b kappa轻链抗体，用于通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和石蜡包埋切片免疫组织化学（IHCP）技术检测小鼠、大鼠和人类中的LKB1（又称STK11或丝氨酸/苏氨酸激酶11）。LKB1单克隆抗体（Ley 37D/G6）靶向重组人LKB1上的表位，确保在各种应用中可靠识别。LKB1是一种重要的丝氨酸/苏氨酸激酶，由433个氨基酸组成，在调节细胞代谢、维持细胞能量平衡和建立细胞极性方面发挥着重要作用。LKB1通过激活AMP激活蛋白激酶（AMPK）和其他相关激酶来发挥抑癌作用，这些激酶对于控制细胞生长和增殖至关重要。LKB1的突变与Peutz-Jeghers综合征（PJS）密切相关，这是一种遗传性疾病，其特征是胃肠道良性息肉、嘴唇色素斑和多种癌症的高风险。了解LKB1的功能对于阐明其在肿瘤抑制和代谢调节中的作用至关重要，这使得抗LKB1抗体（Ley 37D/G6）成为研究癌症生物学和代谢疾病的宝贵工具。LKB1 (Ley 37D/G6) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 变体，为不同的实验需求提供了多种选择。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

LKB1 抗体 (Ley 37D/G6) 参考文献:

  1. LKB1 肿瘤抑制蛋白：细胞极性中的 PARtaker  |  Baas, AF., et al. 2004. Trends Cell Biol. 14: 312-9. PMID: 15183188
  2. LKB1 激酶：新陈代谢和极性的主帅和指挥官。  |  Spicer, J. and Ashworth, A. 2004. Curr Biol. 14: R383-5. PMID: 15186763
  3. LKB1 肿瘤抑制因子对 mTOR 信号进行负向调节。  |  Shaw, RJ., et al. 2004. Cancer Cell. 6: 91-9. PMID: 15261145
  4. IGF-1 以 ATM 依赖性和 LKB1 依赖性方式使 AMPK-α 亚基磷酸化。  |  Suzuki, A., et al. 2004. Biochem Biophys Res Commun. 324: 986-92. PMID: 15485651
  5. Peutz-Jeghers 综合征是由一种新型丝氨酸苏氨酸激酶的突变引起的。  |  Jenne, DE., et al. 1998. Nat Genet. 18: 38-43. PMID: 9425897
  6. Peutz-Jeghers 综合征的丝氨酸/苏氨酸激酶基因缺陷。  |  Hemminki, A., et al. 1998. Nature. 391: 184-7. PMID: 9428765
  7. 散发性乳腺癌中 LKB1/Peutz-Jeghers 综合征基因的体细胞突变频率较低。  |  Bignell, GR., et al. 1998. Cancer Res. 58: 1384-6. PMID: 9537235
  8. 在散发性结直肠和睾丸肿瘤中，LKB1 的体细胞突变非常罕见。  |  Avizienyte, E., et al. 1998. Cancer Res. 58: 2087-90. PMID: 9605748
  9. Peutz-Jeghers 综合征和散发性结肠癌中的 STK11 突变。  |  Resta, N., et al. 1998. Cancer Res. 58: 4799-801. PMID: 9809980
  10. Peutz-Jeghers 综合征中 LKB1 激酶活性的丧失，以及等位基因和基因位点异质性的证据。  |  Mehenni, H., et al. 1998. Am J Hum Genet. 63: 1641-50. PMID: 9837816

订购信息

产品名称产品编号规格价格数量收藏夹

LKB1 抗体 (Ley 37D/G6)

sc-32245
200 µg/ml
$316.00

LKB1 (Ley 37D/G6): m-IgGκ BP-HRP 套装

sc-520825
200 µg Ab, 40 µg BP
$354.00

LKB1 (Ley 37D/G6): m-IgG2b BP-HRP 套装

sc-548856
200 µg Ab; 10 µg BP
$354.00

LKB1 抗体 (Ley 37D/G6) AC

sc-32245 AC
500 µg/ml, 25% agarose
$416.00

LKB1 抗体 (Ley 37D/G6) HRP

sc-32245 HRP
200 µg/ml
$316.00

LKB1 抗体 (Ley 37D/G6) FITC

sc-32245 FITC
200 µg/ml
$330.00

LKB1 抗体 (Ley 37D/G6) PE

sc-32245 PE
200 µg/ml
$343.00

LKB1 抗体 (Ley 37D/G6) Alexa Fluor® 488

sc-32245 AF488
200 µg/ml
$357.00

LKB1 抗体 (Ley 37D/G6) Alexa Fluor® 546

sc-32245 AF546
200 µg/ml
$357.00

LKB1 抗体 (Ley 37D/G6) Alexa Fluor® 594

sc-32245 AF594
200 µg/ml
$357.00

LKB1 抗体 (Ley 37D/G6) Alexa Fluor® 647

sc-32245 AF647
200 µg/ml
$357.00

LKB1 抗体 (Ley 37D/G6) Alexa Fluor® 680

sc-32245 AF680
200 µg/ml
$357.00

LKB1 抗体 (Ley 37D/G6) Alexa Fluor® 790

sc-32245 AF790
200 µg/ml
$357.00

In which region of LKB1 does this antibody bind?

Asked by: sofiabc
Thank you for your question. Anti-LKB1 Antibody (Ley 37D/G6): sc-32245 was raised against recombinant LKB1 of human origin. No epitope mapping was performed for this antibody, so the exact binding site on its target protein is not yet known.
Answered by: Tech Support Europe
Date published: 2022-03-09

I'd really like to know the exact epitope. Is there an update?

Asked by: CKL MZ
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

In which region of LKB1 does this antibody bind?

Asked by: H2019
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined.
Answered by: Technical Support
Date published: 2020-09-14

I would like to use this antibody in tandem with another mouse monoclonal primary antibody for double staining. Is this possible?

Asked by: Trav11
Yes, this is absolutely possible. In this case, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, LKB1 Antibody (Ley 37D/G6): sc-32245 is available conjugated to HRP (sc-32245 HRP), phycoerythrin (sc-32245 PE), fluoroscein (sc-32245 FITC), Alexa Fluor® 488 (sc-32245 AF488) or Alexa Fluor® 647 (sc-32245 AF647). If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Tech Service 8
Date published: 2016-12-30
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Rated 5 out of 5 by from western blotWe used this antibody for Western blot on whole cell lysates. A clean band was showed. Good results.
Date published: 2021-07-22
Rated 5 out of 5 by from Works greatWorks well for western blotting for 1:1000 dilutions.
Date published: 2018-05-16
Rated 5 out of 5 by from fluorescent ICCI got good immunofluorescent images of paraformaldehyde-fixed mouse embryonic fibloblast (MEF) cells using LKB1(Ley37D/G6) antibody under confocal microscope. LKB1 is mainly in nucleus and it translocates to cytoplasm by some stimulus. It is consistent with previous study and analysis with other antibodies from other company It works very well
Date published: 2018-02-01
Rated 5 out of 5 by from greatGood specificity and stripe were seen in immunoblotting, the antibody was diluted at 1:1000 and the results were very good for us.
Date published: 2017-10-31
Rated 5 out of 5 by from Very good antibodyWorked very well. a single band was detected in a human cell lysate
Date published: 2017-10-10
Rated 4 out of 5 by from Good antibodyWorked well with western blotting at 1:500 dilution.
Date published: 2017-09-27
Rated 5 out of 5 by from Excellent!Works really well. My conditions includes - 1:500, MEF cells, RIPA lysis, 30 ug. The band is bright at the predicted size. I am very satisfied.
Date published: 2017-09-21
Rated 3 out of 5 by from just so soI bought this product two months ago.This product is common.
Date published: 2017-09-20
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LKB1 抗体 (Ley 37D/G6) is rated 4.7 out of 5 by 29.
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