LITAF Antibody (C-5): sc-166719

LITAF Antibody (C-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects LITAF protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-LITAF antibody (C-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. LITAF protein, known as lipopolysaccharide-induced tumor necrosis factor alpha factor, plays a pivotal role in regulating inflammatory responses, particularly during lipopolysaccharide (LPS) stimulation. LPS is a potent activator of monocytes and macrophages, leading to secretion of tumor necrosis factor alpha (TNFα) and other inflammatory mediators, which are crucial for immune response to bacterial infections. LITAF is primarily located in the nucleus, where LITAF is essential for transcriptional regulation of TNFα gene, thereby influencing production of this key cytokine. High expression levels of LITAF mRNA have been observed in tissues such as placenta, peripheral blood leukocytes, lymph nodes, and spleen, highlighting LITAF′s importance in immune function and inflammatory processes. LITAF′s interaction with Stat6B and LITAF-Stat6B complex further underscores LITAF′s role in modulating cytokine expression in response to inflammatory stimuli, making LITAF a significant target for research in immunology and inflammation.

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LITAF Antibody (C-5) References:

1. A novel lipopolysaccharide-induced transcription factor regulating tumor necrosis factor alpha gene expression: molecular cloning, sequencing, characterization, and chromosomal assignment.  |  Myokai, F., et al. 1999. Proc Natl Acad Sci U S A. 96: 4518-23. PMID: 10200294
2. Mutation of a putative protein degradation gene LITAF/SIMPLE in Charcot-Marie-Tooth disease 1C.  |  Street, VA., et al. 2003. Neurology. 60: 22-6. PMID: 12525712
3. Kinetics of lipopolysaccharide-induced transcription factor activation/inactivation and relation to proinflammatory gene expression in the murine spleen.  |  Zhou, HR., et al. 2003. Toxicol Appl Pharmacol. 187: 147-61. PMID: 12662898
4. Molecular cloning and characterization of mouse LITAF cDNA: role in the regulation of tumor necrosis factor-alpha (TNF-alpha) gene expression.  |  Bolcato-Bellemin, AL., et al. 2004. J Endotoxin Res. 10: 15-23. PMID: 15025820
5. PIG7/LITAF gene mutation and overexpression of its gene product in extramammary Paget's disease.  |  Matsumura, Y., et al. 2004. Int J Cancer. 111: 218-23. PMID: 15197774
6. LPS induces the interaction of a transcription factor, LPS-induced TNF-alpha factor, and STAT6(B) with effects on multiple cytokines.  |  Tang, X., et al. 2005. Proc Natl Acad Sci U S A. 102: 5132-7. PMID: 15793005
7. A new transcription factor that regulates TNF-alpha gene expression, LITAF, is increased in intestinal tissues from patients with CD and UC.  |  Stucchi, A., et al. 2006. Inflamm Bowel Dis. 12: 581-7. PMID: 16804395
8. Cellular LITAF interacts with frog virus 3 75L protein and alters its subcellular localization.  |  Eaton, HE., et al. 2013. J Virol. 87: 716-23. PMID: 23097445
9. The LITAF/SIMPLE I92V sequence variant results in an earlier age of onset of CMT1A/HNPP diseases.  |  Sinkiewicz-Darol, E., et al. 2015. Neurogenetics. 16: 27-32. PMID: 25342198
10. Kavain Inhibition of LPS-Induced TNF-α via ERK/LITAF.  |  Tang, X. and Amar, S. 2016. Toxicol Res (Camb). 5: 188-196. PMID: 26918116
11. miRNA-106a and prostate cancer radioresistance: a novel role for LITAF in ATM regulation.  |  Hoey, C., et al. 2018. Mol Oncol. 12: 1324-1341. PMID: 29845714
12. Increased Gene Expression of LITAF, TNF-α and BCL6 in Endometrial Tissues of Women with Endometriosis: A Case-Control Study.  |  Saadat Varnosfaderani, A., et al. 2024. Cell J. 26: 243-249. PMID: 38736408

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-LITAF Antibody (C-5) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-LITAF Antibody (C-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

LITAF Antibody (C-5) Specifications

Alternate Product Name: LITAF

Product Clone Name: C-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-161

Epitope Target: LITAF

Epitope Species: human

Product Reactivity/Detection: LITAF

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for LITAF Antibody (C-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin