Lipin-1 Antibody (B-12) recommended for detection of Lipin-1 of mouse, rat and human origin by WB, IP, IF and ELISA

Lipin-1 Antibody (B-12): sc-376874

Lipin-1 Antibody (B-12) is rated 4.7 out of 5 by 9.
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Datasheets

    • Anti-Lipin-1 Antibody (B-12) is a mouse monoclonal IgG1 (kappa light chain) Lipin-1 antibody provided at 200 µg/ml
    • raised against amino acids 261-380 mapping within an internal region of Lipin-1 of human origin
    • Anti-Lipin-1 Antibody (B-12) is recommended for detection of Lipin-1 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-Lipin-1 Antibody (B-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Lipin-1 (B-12): sc-376874.

What is the recommended starting dilution for Western blot for sc-376874: Lipin-1 (B-12) monoclonal antibody?

Asked by: DefinitelyNotMatt
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2019-07-10
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Rated 5 out of 5 by from Good antibody This antoibody is good for western blot. We use triple negative breast cancer cell protein samples, and it shows very strong band.
Date published: 2017-07-09
Rated 5 out of 5 by from expected band I got expected band at 132kda, which is very specific and strong for wb .
Date published: 2017-02-11
Rated 5 out of 5 by from very clear Used this antibody in WB in AN3CA whole cell lysate and got strong band.
Date published: 2017-02-11
Rated 5 out of 5 by from Western Blot data Satisfactory Western Blot data of Lipin-1 expression in A-431 and HeLa whole cell lysate.
Date published: 2017-01-17
Rated 5 out of 5 by from good product! I tested the expression of lipin-1 product in the cytoplasm and nuclear by weston blot using this antibody.In my lab dilution used 1/500.
Date published: 2016-12-25
Rated 5 out of 5 by from lipin-1 is a mouse monoclonal antibody We tested it by weston blot 3 month ago. Its band is clear!
Date published: 2016-12-21
Rated 2 out of 5 by from Prueba en rata En western blot, la detección de Lipina-1 mediante este anticuerpo monoclonal no ha mostrado ninguna banda a la altura esperada en nuestras condiciones de estudio: extracto proteico total de hígado de rata.
Date published: 2016-12-12
Rated 5 out of 5 by from Great antibody This antibody can nicely detect lipin1 protein in transfected cells.
Date published: 2016-11-16
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