LIN-28 Antibody (6D1F9): sc-293120

LIN-28 Antibody (6D1F9) is a mouse monoclonal IgG1 kappa light chain antibody that detects LIN-28 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). LIN-28 (6D1F9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. LIN-28 is a highly conserved, RNA-binding protein located in the cytoplasm, characterized by a cold shock domain and retroviral-type (CCHC) zinc finger motifs, first identified in Caenorhabditis elegans. LIN-28 plays a crucial role in regulating developmental timing during embryogenesis, showing prominent expression in embryos, embryonic stem cells, and embryonal carcinoma cells. LIN-28 expression persists in certain adult tissues, including cardiac and skeletal muscle, where LIN-28 enhances protein synthesis efficiency in differentiating myoblasts and interacts with growth and differentiation factor IGF-II. LIN-28′s ability to modulate these processes highlights significant implications for developmental biology, regenerative medicine, and cancer research.

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LIN-28 Antibody (6D1F9) References:

1. Two genetic circuits repress the Caenorhabditis elegans heterochronic gene lin-28 after translation initiation.  |  Seggerson, K., et al. 2002. Dev Biol. 243: 215-25. PMID: 11884032
2. Conservation of the heterochronic regulator Lin-28, its developmental expression and microRNA complementary sites.  |  Moss, EG. and Tang, L. 2003. Dev Biol. 258: 432-42. PMID: 12798299
3. Temporally regulated expression of Lin-28 in diverse tissues of the developing mouse.  |  Yang, DH. and Moss, EG. 2003. Gene Expr Patterns. 3: 719-26. PMID: 14643679
4. Expression profiling of mammalian microRNAs uncovers a subset of brain-expressed microRNAs with possible roles in murine and human neuronal differentiation.  |  Sempere, LF., et al. 2004. Genome Biol. 5: R13. PMID: 15003116
5. Micro-RNA regulation of the mammalian lin-28 gene during neuronal differentiation of embryonal carcinoma cells.  |  Wu, L. and Belasco, JG. 2005. Mol Cell Biol. 25: 9198-208. PMID: 16227573
6. Identification and characterization of lin-28 homolog B (LIN28B) in human hepatocellular carcinoma.  |  Guo, Y., et al. 2006. Gene. 384: 51-61. PMID: 16971064
7. Multiple mechanisms are involved in regulating the expression of the developmental timing regulator lin-28 in Caenorhabditis elegans.  |  Morita, K. and Han, M. 2006. EMBO J. 25: 5794-804. PMID: 17139256
8. Lin-28 binds IGF-2 mRNA and participates in skeletal myogenesis by increasing translation efficiency.  |  Polesskaya, A., et al. 2007. Genes Dev. 21: 1125-38. PMID: 17473174
9. Lin28 and let-7 in the Metabolic Physiology of Aging.  |  Jun-Hao, ET., et al. 2016. Trends Endocrinol Metab. 27: 132-141. PMID: 26811207
10. Lin28 and let-7: roles and regulation in liver diseases.  |  McDaniel, K., et al. 2016. Am J Physiol Gastrointest Liver Physiol. 310: G757-65. PMID: 27012771
11. The LIN28/let-7 Pathway in Cancer.  |  Balzeau, J., et al. 2017. Front Genet. 8: 31. PMID: 28400788
12. The cold shock domain protein LIN-28 controls developmental timing in C. elegans and is regulated by the lin-4 RNA.  |  Moss, EG., et al. 1997. Cell. 88: 637-46. PMID: 9054503

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-LIN-28 Antibody (6D1F9) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-LIN-28 Antibody (6D1F9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

LIN-28 Antibody (6D1F9) Specifications

Alternate Product Name: LIN-28

Product Clone Name: 6D1F9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 93-209

Epitope Target: LIN-28

Epitope Species: human

Product Reactivity/Detection: LIN-28

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for LIN-28 Antibody (6D1F9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred LIN-28 antibody is LIN-28 Antibody (C-9): sc-374460.