LIMP III Antibody (5A-9): sc-21725
- LIMP III Antibody (5A-9) is a mouse monoclonal IgG2a κ LIMP III antibody provided at 200 µg/ml
- raised against purified cellular fractions from rat liver
- LIMP III Antibody (5A-9) is recommended for detection of LIMP III of mouse and rat origin by WB, IP and IF
- Anti-LIMP III Antibody (5A-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for LIMP III Antibody (5A-9) for WB applications. These reagents are now offered in bundles with LIMP III Antibody (5A-9) (see ordering information below).
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LIMP III Antibody (5A-9) is a mouse monoclonal IgG2a kappa light chain antibody that detects LIMP III protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-LIMP III antibody (5A-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. LIMP III plays a crucial role in the lysosomal membrane, where LIMP III is involved in maintaining lysosomal integrity and function. LIMP III is essential for proper trafficking of lysosomal enzymes and substrates, which are vital for cellular waste management and recycling processes. LIMP III localization within lysosomes ensures effective degradation and recycling of cellular components, preventing accumulation of damaged or unnecessary proteins. Additionally, lysosomal integral membrane proteins, including LIMP III, serve as critical markers for lysosomes, making LIMP III invaluable for microscopy studies, cellular fractionation validation, and research focused on protein trafficking through the secretory pathway. LIMP III (5A-9) monoclonal antibody enables researchers to gain insights into lysosomal function and its implications in various cellular processes and diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
LIMP III Antibody (5A-9) References:
- Bi-directional trafficking between the trans-Golgi network and the endosomal/lysosomal system. | Rohn, WM., et al. 2000. J Cell Sci. 113 (Pt 12): 2093-101. PMID: 10825282
- Targeting of lysosomal integral membrane protein LIMP II. The tyrosine-lacking carboxyl cytoplasmic tail of LIMP II is sufficient for direct targeting to lysosomes. | Vega, MA., et al. 1991. J Biol Chem. 266: 16269-72. PMID: 1715864
- The lysosomal proenzyme receptor that binds procathepsin L to microsomal membranes at pH 5 is a 43-kDa integral membrane protein. | McIntyre, GF. and Erickson, AH. 1993. Proc Natl Acad Sci U S A. 90: 10588-92. PMID: 8248149
- The tyrosine-based lysosomal targeting signal in lamp-1 mediates sorting into Golgi-derived clathrin-coated vesicles. | Höning, S., et al. 1996. EMBO J. 15: 5230-9. PMID: 8895568
- Lysosomal integral membrane protein II binds thrombospondin-1. Structure-function homology with the cell adhesion molecule CD36 defines a conserved recognition motif. | Crombie, R. and Silverstein, R. 1998. J Biol Chem. 273: 4855-63. PMID: 9478926
- The mammalian AP-3 adaptor-like complex mediates the intracellular transport of lysosomal membrane glycoproteins. | Le Borgne, R., et al. 1998. J Biol Chem. 273: 29451-61. PMID: 9792650
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LIMP III Antibody (5A-9) | sc-21725 | 200 µg/ml | $322.00 | |||
LIMP III Antibody (5A-9): m-IgG Fc BP-HRP Bundle | sc-528364 | 200 µg Ab; 10 µg BP | $361.00 | |||
LIMP III Antibody (5A-9): m-IgGκ BP-HRP Bundle | sc-520725 | 200 µg Ab, 40 µg BP | $361.00 | |||
LIMP III Antibody (5A-9): m-IgG2a BP-HRP Bundle | sc-547078 | 200 µg Ab; 10 µg BP | $361.00 | |||
LIMP III Antibody (5D-9) AC | sc-21725 AC | 500 µg/ml, 25% agarose | $424.00 | |||
LIMP III Antibody (5D-9) HRP | sc-21725 HRP | 200 µg/ml | $322.00 | |||
LIMP III Antibody (5D-9) FITC | sc-21725 FITC | 200 µg/ml | $336.00 | |||
LIMP III Antibody (5D-9) PE | sc-21725 PE | 200 µg/ml | $349.00 | |||
LIMP III Antibody (5D-9) Alexa Fluor® 488 | sc-21725 AF488 | 200 µg/ml | $364.00 | |||
LIMP III Antibody (5A-9) Alexa Fluor® 546 | sc-21725 AF546 | 200 µg/ml | $364.00 | |||
LIMP III Antibody (5D-9) Alexa Fluor® 594 | sc-21725 AF594 | 200 µg/ml | $364.00 | |||
LIMP III Antibody (5D-9) Alexa Fluor® 647 | sc-21725 AF647 | 200 µg/ml | $364.00 | |||
LIMP III Antibody (5D-9) Alexa Fluor® 680 | sc-21725 AF680 | 200 µg/ml | $364.00 | |||
LIMP III Antibody (5D-9) Alexa Fluor® 790 | sc-21725 AF790 | 200 µg/ml | $364.00 |