Limd1 Antibody (H-4): sc-271448

Limd1 Antibody (H-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects Limd1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Limd1 antibody (H-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Limd1, also known as LIM domain-containing protein 1, plays a crucial role as a tumor suppressor and is characterized by three LIM zinc-binding domains, which are essential for mediating protein-protein interactions. These LIM domains consist of a cysteine-rich consensus sequence that enables Limd1 to interact with various partners, including SQSTM1, Rb, p62, and TRAF6. Limd1 regulates cellular processes such as osteoclast development under stress and contributes to tumor growth inhibition, showing down-regulation in lung cancer and deletion in some cervical cancers. Understanding Limd1 interactions and functions provides insights into potential therapeutic targets in cancer treatment and other diseases.

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Limd1 Antibody (H-4) References:

1. A novel gene containing LIM domains (LIMD1) is located within the common eliminated region 1 (C3CER1) in 3p21.3.  |  Kiss, H., et al. 1999. Hum Genet. 105: 552-9. PMID: 10647888
2. Inactivation of the human fragile histidine triad gene at 3p14.2 in monochromosomal human/mouse microcell hybrid-derived severe combined immunodeficient mouse tumors.  |  Kholodnyuk, ID., et al. 2000. Cancer Res. 60: 7119-25. PMID: 11156420
3. The LZTFL1 gene is a part of a transcriptional map covering 250 kb within the common eliminated region 1 (C3CER1) in 3p21.3.  |  Kiss, H., et al. 2001. Genomics. 73: 10-9. PMID: 11352561
4. LIM domains-containing protein 1 (LIMD1), a tumor suppressor encoded at chromosome 3p21.3, binds pRB and represses E2F-driven transcription.  |  Sharp, TV., et al. 2004. Proc Natl Acad Sci U S A. 101: 16531-6. PMID: 15542589
5. The tumor suppressor Scrib interacts with the zyxin-related protein LPP, which shuttles between cell adhesion sites and the nucleus.  |  Petit, MM., et al. 2005. BMC Cell Biol. 6: 1. PMID: 15649318
6. The LIM protein Ajuba influences interleukin-1-induced NF-kappaB activation by affecting the assembly and activity of the protein kinase Czeta/p62/TRAF6 signaling complex.  |  Feng, Y. and Longmore, GD. 2005. Mol Cell Biol. 25: 4010-22. PMID: 15870274
7. The tumor suppressor Scrib selectively interacts with specific members of the zyxin family of proteins.  |  Petit, MM., et al. 2005. FEBS Lett. 579: 5061-8. PMID: 16137684
8. The LIM protein, Limd1, regulates AP-1 activation through an interaction with Traf6 to influence osteoclast development.  |  Feng, Y., et al. 2007. J Biol Chem. 282: 39-48. PMID: 17092936
9. Modeling non-random deletions in cancer.  |  Kost-Alimova, M. and Imreh, S. 2007. Semin Cancer Biol. 17: 19-30. PMID: 17174104

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Limd1 Antibody (H-4) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-Limd1 Antibody (H-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Limd1 Antibody (H-4) Specifications

Alternate Product Name: Limd1

Product Clone Name: H-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-57

Epitope Target: Limd1

Epitope Species: human

Product Reactivity/Detection: Limd1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for Limd1 Antibody (H-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin