Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
LHX4 CRISPR/Cas9 KO Plasmid (m): sc-421427
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LHX4 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the LHX4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LHX4 Antibody (E-10): sc-374562
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LHX4 CRISPR/Cas9 KO Plasmid (m) | sc-421427 | 20 µg | $397.00 |
Overview
Lhx4 encodes LIM homeobox protein 4 (LHX4), a transcription factor that binds DNA through a homeodomain and coordinates gene expression programs critical for embryonic patterning and organogenesis. In mouse development, LHX4 contributes to specification and differentiation of anterior pituitary lineages and broader neuronal developmental processes by integrating with LIM-domain cofactors and homeobox transcriptional networks. Its activity influences cell fate decisions, lineage commitment, and morphogen-responsive transcriptional regulation during tissue formation. Dysregulation of LHX4-associated developmental pathways is relevant to research on pituitary and neurodevelopmental phenotypes, where altered transcription factor dosage can perturb endocrine axis formation and neural circuit establishment.
LHX4 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Lhx4 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Lhx4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Lhx4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish LHX4 protein expression.
This CRISPR knockout system enables efficient generation of Lhx4-deficient cell models for investigation of LHX4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Lhx4 exon(s) critical for LHX4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Lhx4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by LHX4 CRISPR/Cas9 KO Plasmid (m) and LHX4 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Lhx4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by LHX4 HDR Plasmid (m) and LHX4 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Lhx4 homology arms to support homology-directed repair at defined Lhx4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.