LHX1 Antibody (C-6): sc-515631
- LHX1 Antibody (C-6) is a mouse monoclonal IgG2a κ LHX1 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 121-163 mapping within an internal region of LHX1 of human origin
- LHX1 Antibody (C-6) is recommended for detection of LHX1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-LHX1 Antibody (C-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-515631 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for LHX1 Antibody (C-6) for WB applications. These reagents are now offered in bundles with LHX1 Antibody (C-6) (see ordering information below).
LHX1 Antibody (C-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects LHX1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). LHX1 (C-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. LHX1 protein plays a crucial role in motor neuron development by regulating cell migration and guiding motor axons, which is essential for establishing correct axonal pathway trajectory. This regulation is vital for proper motor neuron subtype formation, as distinct combinations of LIM-type homeodomain factors, including LHX1, are expressed during genetically diverse motor neuron development. Human LHX1 gene, located on chromosome 11p13-p12, encodes a 384 amino acid protein and consists of five exons separated by introns of varying lengths. Notably, two LIM domains are found entirely within first and second exons, while homeodomain is divided between third and fourth exons. LHX1′s intricate structure, with LIM and homeodomains, is essential for neuronal development function, highlighting LHX1′s importance in the nervous system.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
LHX1 Antibody (C-6) References:
- The LIM homeodomain protein dLim1 defines a subclass of neurons within the embryonic ventral nerve cord of Drosophila. | Lilly, B., et al. 1999. Mech Dev. 88: 195-205. PMID: 10534618
- Requirement of LIM domains for LIM1 function in mouse head development. | Cheah, SS., et al. 2000. Genesis. 27: 12-21. PMID: 10862151
- Genetic and epigenetic mechanisms contribute to motor neuron pathfinding. | Sharma, K., et al. 2000. Nature. 406: 515-9. PMID: 10952312
- Loss of Lhx1 activity impacts on the localization of primordial germ cells in the mouse. | Tanaka, SS., et al. 2010. Dev Dyn. 239: 2851-9. PMID: 20845430
- Frame shift mutation of LHX1 is associated with Mayer-Rokitansky-Kuster-Hauser (MRKH) syndrome. | Ledig, S., et al. 2012. Hum Reprod. 27: 2872-5. PMID: 22740494
- Lhx1 is required in Müllerian duct epithelium for uterine development. | Huang, CC., et al. 2014. Dev Biol. 389: 124-36. PMID: 24560999
- Lhx1 maintains synchrony among circadian oscillator neurons of the SCN. | Hatori, M., et al. 2014. Elife. 3: e03357. PMID: 25035422
- Lhx1 functions together with Otx2, Foxa2, and Ldb1 to govern anterior mesendoderm, node, and midline development. | Costello, I., et al. 2015. Genes Dev. 29: 2108-22. PMID: 26494787
- An LHX1-Regulated Transcriptional Network Controls Sleep/Wake Coupling and Thermal Resistance of the Central Circadian Clockworks. | Bedont, JL., et al. 2017. Curr Biol. 27: 128-136. PMID: 28017605
- The islet-expressed Lhx1 transcription factor interacts with Islet-1 and contributes to glucose homeostasis. | Bethea, M., et al. 2019. Am J Physiol Endocrinol Metab. 316: E397-E409. PMID: 30620636
- The exon-intron structure of human LHX1 gene. | Bozzi, F., et al. 1996. Biochem Biophys Res Commun. 229: 494-7. PMID: 8954926
- The nuclear LIM domain interactor NLI mediates homo- and heterodimerization of LIM domain transcription factors. | Jurata, LW., et al. 1998. J Biol Chem. 273: 3152-7. PMID: 9452425
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LHX1 Antibody (C-6) | sc-515631 | 200 µg/ml | $322.00 | |||
LHX1 Antibody (C-6): m-IgG Fc BP-HRP Bundle | sc-531449 | 200 µg Ab; 10 µg BP | $361.00 | |||
LHX1 Antibody (C-6): m-IgGκ BP-HRP Bundle | sc-525139 | 200 µg Ab, 40 µg BP | $361.00 | |||
LHX1 Antibody (C-6): m-IgG2a BP-HRP Bundle | sc-547790 | 200 µg Ab; 10 µg BP | $361.00 | |||
LHX1 Antibody (C-6) X | sc-515631 X | 200 µg/0.1 ml | $322.00 | |||
LHX1 Antibody (C-6) AC | sc-515631 AC | 500 µg/ml, 25% agarose | $424.00 | |||
LHX1 Antibody (C-6) HRP | sc-515631 HRP | 200 µg/ml | $322.00 | |||
LHX1 Antibody (C-6) FITC | sc-515631 FITC | 200 µg/ml | $336.00 | |||
LHX1 Antibody (C-6) PE | sc-515631 PE | 200 µg/ml | $349.00 | |||
LHX1 Antibody (C-6) Alexa Fluor® 488 | sc-515631 AF488 | 200 µg/ml | $364.00 | |||
LHX1 Antibody (C-6) Alexa Fluor® 546 | sc-515631 AF546 | 200 µg/ml | $364.00 | |||
LHX1 Antibody (C-6) Alexa Fluor® 594 | sc-515631 AF594 | 200 µg/ml | $364.00 | |||
LHX1 Antibody (C-6) Alexa Fluor® 647 | sc-515631 AF647 | 200 µg/ml | $364.00 | |||
LHX1 Antibody (C-6) Alexa Fluor® 680 | sc-515631 AF680 | 200 µg/ml | $364.00 | |||
LHX1 Antibody (C-6) Alexa Fluor® 790 | sc-515631 AF790 | 200 µg/ml | $364.00 |