Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
LETM1 CRISPR/Cas9 KO Plasmid (h): sc-406163
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LETM1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the LETM1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LETM1 Antibody (D-5): sc-271235
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LETM1 CRISPR/Cas9 KO Plasmid (h) | sc-406163 | 20 µg | $397.00 |
Overview
LETM1 (leucine zipper EF-hand containing transmembrane protein 1) is an inner mitochondrial membrane protein implicated in mitochondrial ion homeostasis and bioenergetic control. It contributes to regulation of mitochondrial morphology, cristae organization, and coupling of ion transport to oxidative phosphorylation, linking LETM1 activity to cellular energy metabolism and stress responses. Altered LETM1 dosage has been associated with neurodevelopmental phenotypes and mitochondrial dysfunction, and dysregulation has been explored in the context of tumor cell metabolism and proliferation. As a mitochondrial regulator, LETM1 is frequently studied for its impact on apoptosis sensitivity, reactive oxygen species signaling, and metabolic pathway remodeling.
LETM1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the LETM1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the LETM1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the LETM1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish LETM1 protein expression.
This CRISPR knockout system enables efficient generation of LETM1-deficient cell models for investigation of LETM1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting LETM1 exon(s) critical for LETM1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple LETM1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by LETM1 CRISPR/Cas9 KO Plasmid (h) and LETM1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the LETM1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by LETM1 HDR Plasmid (h) and LETM1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by LETM1 homology arms to support homology-directed repair at defined LETM1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.