LETM1 Antibody (D-5): sc-271235
- LETM1 Antibody (D-5) is a mouse monoclonal IgG1 κ LETM1 antibody provided at 200 µg/ml
- raised against amino acids 440-739 mapping at the C-terminus of LETM1 of human origin
- LETM1 Antibody (D-5) is recommended for detection of LETM1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-LETM1 Antibody (D-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for LETM1 Antibody (D-5) for WB and IHC(P) applications. This reagent is now offered in a bundle with LETM1 Antibody (D-5) (see ordering information below).
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LETM1 Antibody (D-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects LETM1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-LETM1 antibody (D-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. LETM1, or leucine zipper-EF-hand-containing transmembrane protein 1, mitochondrial, is a 739 amino acid protein primarily localized to the mitochondrial membrane, where LETM1 plays a crucial role in maintaining mitochondrial function and overall cellular health. LETM1 features a leucine zipper motif, a transmembrane domain, and two EF-hand calcium-binding domains, which are essential for potential function as a calcium-binding protein. Phosphorylation sites within LETM1 suggest possible post-translational modifications that could regulate activity and interactions. Deletions in the gene encoding LETM1 have been linked to Wolf-Hirschhorn syndrome, a congenital disorder characterized by various developmental abnormalities, including cognitive impairment and organ malformations, highlighting LETM1′s importance in normal physiological processes. Human LETM1 shares 84% similarity with mouse LETM1, indicating a conserved function across species, making LETM1 (D-5) antibody a valuable tool for studying mitochondrial dynamics and related pathologies in diverse biological contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
LETM1 Antibody (D-5) References:
- LETM1, a novel gene encoding a putative EF-hand Ca(2+)-binding protein, flanks the Wolf-Hirschhorn syndrome (WHS) critical region and is deleted in most WHS patients. | Endele, S., et al. 1999. Genomics. 60: 218-25. PMID: 10486213
- First known microdeletion within the Wolf-Hirschhorn syndrome critical region refines genotype-phenotype correlation. | Rauch, A., et al. 2001. Am J Med Genet. 99: 338-42. PMID: 11252005
- Mapping the Wolf-Hirschhorn syndrome phenotype outside the currently accepted WHS critical region and defining a new critical region, WHSCR-2. | Zollino, M., et al. 2003. Am J Hum Genet. 72: 590-7. PMID: 12563561
- LETM1, a gene deleted in Wolf-Hirschhorn syndrome, encodes an evolutionarily conserved mitochondrial protein. | Schlickum, S., et al. 2004. Genomics. 83: 254-61. PMID: 14706454
- The LETM1/YOL027 gene family encodes a factor of the mitochondrial K+ homeostasis with a potential role in the Wolf-Hirschhorn syndrome. | Nowikovsky, K., et al. 2004. J Biol Chem. 279: 30307-15. PMID: 15138253
- Inverse correlation between expression of the Wolfs Hirschhorn candidate gene Letm1 and mitochondrial volume in C. elegans and in mammalian cells. | Hasegawa, A. and van der Bliek, AM. 2007. Hum Mol Genet. 16: 2061-71. PMID: 17606466
- Two unique patients with novel microdeletions in 4p16.3 that exclude the WHS critical regions: implications for critical region designation. | South, ST., et al. 2007. Am J Med Genet A. 143A: 2137-42. PMID: 17696124
- LETM1, deleted in Wolf-Hirschhorn syndrome is required for normal mitochondrial morphology and cellular viability. | Dimmer, KS., et al. 2008. Hum Mol Genet. 17: 201-14. PMID: 17925330
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LETM1 Antibody (D-5) | sc-271235 | 200 µg/ml | $322.00 | |||
LETM1 Antibody (D-5): m-IgG Fc BP-HRP Bundle | sc-525792 | 200 µg Ab; 10 µg BP | $361.00 | |||
LETM1 Antibody (D-5) AC | sc-271235 AC | 500 µg/ml, 25% agarose | $424.00 | |||
LETM1 Antibody (D-5) HRP | sc-271235 HRP | 200 µg/ml | $322.00 | |||
LETM1 Antibody (D-5) FITC | sc-271235 FITC | 200 µg/ml | $336.00 | |||
LETM1 Antibody (D-5) PE | sc-271235 PE | 200 µg/ml | $349.00 | |||
LETM1 Antibody (D-5) Alexa Fluor® 488 | sc-271235 AF488 | 200 µg/ml | $364.00 | |||
LETM1 Antibody (D-5) Alexa Fluor® 546 | sc-271235 AF546 | 200 µg/ml | $364.00 | |||
LETM1 Antibody (D-5) Alexa Fluor® 594 | sc-271235 AF594 | 200 µg/ml | $364.00 | |||
LETM1 Antibody (D-5) Alexa Fluor® 647 | sc-271235 AF647 | 200 µg/ml | $364.00 | |||
LETM1 Antibody (D-5) Alexa Fluor® 680 | sc-271235 AF680 | 200 µg/ml | $364.00 | |||
LETM1 Antibody (D-5) Alexa Fluor® 790 | sc-271235 AF790 | 200 µg/ml | $364.00 |