LEF-1 Antibody (B-6): sc-374522
- LEF-1 Antibody (B-6) is a mouse monoclonal IgG1 κ, cited in 15 publications, provided at 200 µg/ml
- raised against amino acids 61-130 mapping near the N-terminus of LEF-1 of human origin
- Anti-LEF-1 Antibody (B-6) is recommended for detection of LEF-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-LEF-1 Antibody (B-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-374522 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for LEF-1 Antibody (B-6) for WB and IHC(P) applications. This reagent is now offered in a bundle with LEF-1 Antibody (B-6) (see ordering information below).
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SEE ALSO...
LEF-1 Antibody (B-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects LEF-1 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-LEF-1 antibody (B-6) is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. LEF-1, a member of the TCF (T cell factor) family of transcription factors, plays a crucial role in regulating gene expression during development, particularly in lymphoid and neuronal cells. LEF-1 is characterized by a high mobility group (HMG) box, which enables DNA-binding capability. This structural feature allows LEF-1 to interact with β-catenin in the nucleus, forming complexes that activate Wnt target genes, which are vital for various cellular processes including cell proliferation and differentiation. Dysregulation of this pathway, often due to mutations in the adenomatous polyposis coli (APC) gene, can lead to β-catenin accumulation and aberrant activation of LEF-1 target genes, contributing to oncogenesis, particularly in colorectal cancer. LEF-1 (B-6) antibody′s availability in multiple formats enhances experimental versatility, making this tool essential for researchers studying Wnt signaling and its implications in development and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Contains Mercury: All HRP conjugates (including bundle forms) contain thiomersal, a mercury compound. Do not dispose in trash. Dispose as hazardous chemical waste according to local, state and federal regulations.
Download thiomersal SDS
LEF-1 Antibody (B-6) References:
- Regulation of LEF-1/TCF transcription factors by Wnt and other signals. | Eastman, Q. and Grosschedl, R. 1999. Curr Opin Cell Biol. 11: 233-40. PMID: 10209158
- LEF-1 regulates proliferation and MMP-7 transcription in breast cancer cells. | Bucan, V., et al. 2012. Genes Cells. 17: 559-67. PMID: 22686279
- Interactions among HCLS1, HAX1 and LEF-1 proteins are essential for G-CSF-triggered granulopoiesis. | Skokowa, J., et al. 2012. Nat Med. 18: 1550-9. PMID: 23001182
- LEF-1 drives aberrant β-catenin nuclear localization in myeloid leukemia cells. | Morgan, RG., et al. 2019. Haematologica. 104: 1365-1377. PMID: 30630973
- DOCK2 couples with LEF-1 to regulate B cell metabolism and memory response. | Yang, L., et al. 2020. Biochem Biophys Res Commun. 529: 296-302. PMID: 32703426
- Lef-1 controls cell cycle progression in airway basal cells to regulate proliferation and differentiation. | Jensen-Cody, CW., et al. 2021. Stem Cells. 39: 1221-1235. PMID: 33932322
- The protective mechanism of SIRT1 on cartilage through regulation of LEF-1. | Hu, X., et al. 2021. BMC Musculoskelet Disord. 22: 642. PMID: 34315467
- LEF1 enhances β-catenin transactivation through IDR-dependent liquid-liquid phase separation. | Zhao, B., et al. 2023. Life Sci Alliance. 6: PMID: 37657935
- ZMIZ2 facilitates hepatocellular carcinoma progression via LEF1 mediated activation of Wnt/β-catenin pathway. | Ding, Y., et al. 2024. Exp Hematol Oncol. 13: 5. PMID: 38254216
- Induction of a beta-catenin-LEF-1 complex by wnt-1 and transforming mutants of beta-catenin. | Porfiri, E., et al. 1997. Oncogene. 15: 2833-9. PMID: 9419974
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LEF-1 Antibody (B-6) | sc-374522 | 200 µg/ml | $322.00 | |||
LEF-1 Antibody (B-6): m-IgG Fc BP-HRP Bundle | sc-525974 | 200 µg Ab; 10 µg BP | $361.00 | |||
LEF-1 Antibody (B-6) X | sc-374522 X | 200 µg/0.1 ml | $322.00 | |||
LEF-1 Antibody (B-6) AC | sc-374522 AC | 500 µg/ml, 25% agarose | $424.00 | |||
LEF-1 Antibody (B-6) HRP | sc-374522 HRP | 200 µg/ml | $322.00 | |||
LEF-1 Antibody (B-6) FITC | sc-374522 FITC | 200 µg/ml | $336.00 | |||
LEF-1 Antibody (B-6) PE | sc-374522 PE | 200 µg/ml | $349.00 | |||
LEF-1 Antibody (B-6) Alexa Fluor® 488 | sc-374522 AF488 | 200 µg/ml | $364.00 | |||
LEF-1 Antibody (B-6) Alexa Fluor® 546 | sc-374522 AF546 | 200 µg/ml | $364.00 | |||
LEF-1 Antibody (B-6) Alexa Fluor® 594 | sc-374522 AF594 | 200 µg/ml | $364.00 | |||
LEF-1 Antibody (B-6) Alexa Fluor® 647 | sc-374522 AF647 | 200 µg/ml | $364.00 | |||
LEF-1 Antibody (B-6) Alexa Fluor® 680 | sc-374522 AF680 | 200 µg/ml | $364.00 | |||
LEF-1 Antibody (B-6) Alexa Fluor® 790 | sc-374522 AF790 | 200 µg/ml | $364.00 |