Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
LDH-B CRISPR/Cas9 KO Plasmid (h): sc-416429
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LDH-B CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the LDH-B genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LDH-B Antibody (431.1): sc-100775
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LDH-B CRISPR/Cas9 KO Plasmid (h) | sc-416429 | 20 µg | $397.00 |
Overview
LDHB encodes the LDH-B subunit of lactate dehydrogenase, a key cytosolic enzyme that catalyzes the interconversion of lactate and pyruvate with concomitant NADH/NAD⁺ cycling. By regulating pyruvate utilization and lactate clearance, LDH-B helps coordinate glycolysis, mitochondrial oxidative metabolism, and redox homeostasis, influencing energy production under variable oxygen and nutrient conditions. LDHB activity contributes to metabolic rewiring during cellular stress and differentiation, and altered LDH isoenzyme balance has been linked to phenotypes associated with hypoxia responses and proliferative metabolism in diverse disease-relevant contexts. As a metabolic node, LDH-B is frequently examined alongside glycolytic control points, mitochondrial function, and lactate signaling in mechanistic studies.
LDH-B CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the LDHB gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the LDHB together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the LDHB open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish LDH-B protein expression.
This CRISPR knockout system enables efficient generation of LDHB-deficient cell models for investigation of LDH-B signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting LDHB exon(s) critical for LDH-B function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple LDHB genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by LDH-B CRISPR/Cas9 KO Plasmid (h) and LDH-B CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the LDHB locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by LDH-B HDR Plasmid (h) and LDH-B HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by LDHB homology arms to support homology-directed repair at defined LDHB target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.