LAT1 Antibody (D-10): sc-374232

LAT1 Antibody (D-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects LAT1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). LAT1 (D-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The L-type amino acid transporter 1 (LAT1) is a crucial multipass-membrane protein that facilitates the sodium-independent, high-affinity transport of large neutral amino acids, functioning as a disulfide-linked heterodimer with the amino acid transport protein CD98. LAT1 is predominantly expressed in adult lung and liver tissues, but is also found in the brain, thymus, retina, testis, placenta, bone marrow, and fetal liver. LAT1 localizes to the blood-retinal barrier, where LAT1 plays a vital role in mediating the transport of L-leucine from the bloodstream to the retina, essential for maintaining retinal health and function. LAT1′s increased expression at the blood-brain barrier in response to elevated phenylalanine levels is linked to neurological complications associated with phenylketonuria. LAT1 transports the amino-acid-related anticancer agent melphalan, highlighting LAT1′s significant involvement in cell proliferation, differentiation, and invasion, particularly in esophageal squamous cell carcinoma.

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LAT1 Antibody (D-10) References:

1. Human L-type amino acid transporter 1 (LAT1): characterization of function and expression in tumor cell lines.  |  Yanagida, O., et al. 2001. Biochim Biophys Acta. 1514: 291-302. PMID: 11557028
2. Expression of L-type amino acid transporter 1 in a rat model of liver metastasis: positive correlation with tumor size.  |  Tamai, S., et al. 2001. Cancer Detect Prev. 25: 439-45. PMID: 11718450
3. Expression of L-type amino acid transporter 1 (LAT1) and 4F2 heavy chain (4F2hc) in liver tumor lesions of rat models.  |  Ohkame, H., et al. 2001. J Surg Oncol. 78: 265-71; discussion 271-2. PMID: 11745822
4. Transport of amino acid-related compounds mediated by L-type amino acid transporter 1 (LAT1): insights into the mechanisms of substrate recognition.  |  Uchino, H., et al. 2002. Mol Pharmacol. 61: 729-37. PMID: 11901210
5. Human LAT1 single nucleotide polymorphism N230K does not alter phenylalanine transport.  |  Boado, RJ., et al. 2004. Mol Genet Metab. 83: 306-11. PMID: 15589117
6. Identification of stereoselective transporters for S-nitroso-L-cysteine: role of LAT1 and LAT2 in biological activity of S-nitrosothiols.  |  Li, S. and Whorton, AR. 2005. J Biol Chem. 280: 20102-10. PMID: 15769744
7. Expression of L-type amino acid transporter 1 (LAT1) in esophageal carcinoma.  |  Kobayashi, H., et al. 2005. J Surg Oncol. 90: 233-8. PMID: 15906366
8. L-type amino acid transporter 1-mediated L-leucine transport at the inner blood-retinal barrier.  |  Tomi, M., et al. 2005. Invest Ophthalmol Vis Sci. 46: 2522-30. PMID: 15980244
9. Structure of the human LAT1-4F2hc heteromeric amino acid transporter complex.  |  Yan, R., et al. 2019. Nature. 568: 127-130. PMID: 30867591
10. Amino acid transporter LAT1 (SLC7A5) as a molecular target for cancer diagnosis and therapeutics.  |  Kanai, Y. 2022. Pharmacol Ther. 230: 107964. PMID: 34390745
11. The complete assembly of human LAT1-4F2hc complex provides insights into its regulation, function and localisation.  |  Wu, D., et al. 2024. Nat Commun. 15: 3711. PMID: 38697966
12. Involvement of L-type-like amino acid transporters in S-nitrosocysteine-stimulated noradrenaline release in the rat hippocampus.  |  Satoh, S., et al. 1997. J Neurochem. 69: 2197-205. PMID: 9349567

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-LAT1 Antibody (D-10) has 33 citations in a variety of scientific publications.

Primary antibodies like Anti-LAT1 Antibody (D-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

LAT1 Antibody (D-10) Specifications

Alternate Product Name: SLC7A5

Product Clone Name: D-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 365-507

Epitope Target: LAT1

Epitope Species: human

Product Reactivity/Detection: LAT1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 33 citations in various scientific publications

Available Conjugates/Forms for LAT1 Antibody (D-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin