LAT Antibody (H-12): sc-393364

LAT Antibody (H-12) is a mouse monoclonal IgG1 antibody that detects LAT in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). LAT, or linker for activation of T cells, is a crucial integral membrane protein that plays a significant role in T cell activation by serving as a scaffold for various signaling molecules. Upon T cell receptor engagement, LAT becomes phosphorylated by ZAP-70/Syk tyrosine kinases, facilitating the recruitment of key proteins such as phospholipase C gamma 1, GRB2, and the p85 subunit of phosphoinositide 3-kinase. This interaction is vital for the downstream signaling cascade that leads to T cell proliferation and differentiation. Notably, LAT is palmitoylated, a post-translational modification that is essential for proper localization to glycolipid-enriched microdomains within the cell membrane, which enhances signaling capabilities. LAT antibody (H-12) is an invaluable tool for researchers studying T cell signaling pathways and their implications in immune responses.

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LAT Antibody (H-12) References:

1. Potentiation of CD3-induced expression of the linker for activation of T cells (LAT) by the calcineurin inhibitors cyclosporin A and FK506.  |  Peters, D., et al. 2000. Blood. 95: 2733-41. PMID: 10779414
2. Analysis of the molecular mechanism involved in 2B4-mediated NK cell activation: evidence that human 2B4 is physically and functionally associated with the linker for activation of T cells.  |  Bottino, C., et al. 2000. Eur J Immunol. 30: 3718-22. PMID: 11169415
3. Mapping the Zap-70 phosphorylation sites on LAT (linker for activation of T cells) required for recruitment and activation of signalling proteins in T cells.  |  Paz, PE., et al. 2001. Biochem J. 356: 461-71. PMID: 11368773
4. Knock-in mutation of the distal four tyrosines of linker for activation of T cells blocks murine T cell development.  |  Sommers, CL., et al. 2001. J Exp Med. 194: 135-42. PMID: 11457888
5. Control of TCR-mediated activation of beta 1 integrins by the ZAP-70 tyrosine kinase interdomain B region and the linker for activation of T cells adapter protein.  |  Goda, S., et al. 2004. J Immunol. 172: 5379-87. PMID: 15100278
6. Linker for activation of B cells: a functional equivalent of a mutant linker for activation of T cells deficient in phospholipase C-gamma1 binding.  |  Janssen, E., et al. 2004. J Immunol. 172: 6810-9. PMID: 15153499
7. In situ expression of LAT (linker for activation of T cells) in pathological human skin with T-lymphoid infiltrate.  |  Alaibac, M., et al. 2004. Arch Dermatol Res. 296: 231-4. PMID: 15449076
8. Palmitoylation and intracellular domain interactions both contribute to raft targeting of linker for activation of T cells.  |  Shogomori, H., et al. 2005. J Biol Chem. 280: 18931-42. PMID: 15753089
9. Platelet endothelial cell adhesion molecule-1 regulates collagen-stimulated platelet function by modulating the association of phosphatidylinositol 3-kinase with Grb-2-associated binding protein-1 and linker for activation of T cells.  |  Moraes, LA., et al. 2010. J Thromb Haemost. 8: 2530-41. PMID: 20723025
10. Assessment of caspase mediated degradation of linker for activation of T cells (LAT) at a single cell level.  |  Kłossowicz, M., et al. 2013. J Immunol Methods. 389: 9-17. PMID: 23261919

The preferred LAT antibody is LAT Antibody (11B.12): sc-53550.