LASP-1 Antibody (G-7): sc-374059

LASP-1 Antibody (G-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects LASP-1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). LASP-1 Antibody (G-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. LASP-1, also known as LIM and SH3 domain protein 1 or MLN50, is a 261 amino acid protein that plays a crucial role in cytoskeletal organization by localizing to both the cytoplasm and cytoskeleton. LASP-1 contains one SH3 domain, one LIM zinc-binding domain, and two nebulin repeats, which are essential for interaction with F-Actin. This interaction maintains structural integrity and dynamic nature of the cytoskeleton, important for various cellular processes, including cell shape, motility, and division. LASP-1 undergoes post-translational modifications such as phosphorylation, which influences function and interactions, particularly in ion transport activities in epithelial cells. Overexpression of LASP-1 has been linked to breast cancer, indicating potential role in tumor transformation and metastasis. LASP-1 gene is located on human chromosome 17, a region encompassing over 2.5% of the human genome and containing more than 1,200 genes, highlighting significance in both normal physiology and disease states.

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LASP-1 Antibody (G-7) References:

1. The human LASP1 gene is fused to MLL in an acute myeloid leukemia with t(11;17)(q23;q21).  |  Strehl, S., et al. 2003. Oncogene. 22: 157-60. PMID: 12527918
2. Actin binding of human LIM and SH3 protein is regulated by cGMP- and cAMP-dependent protein kinase phosphorylation on serine 146.  |  Butt, E., et al. 2003. J Biol Chem. 278: 15601-7. PMID: 12571245
3. Zyxin interacts with the SH3 domains of the cytoskeletal proteins LIM-nebulette and Lasp-1.  |  Li, B., et al. 2004. J Biol Chem. 279: 20401-10. PMID: 15004028
4. Phosphorylation of mouse LASP-1 on threonine 156 by cAMP- and cGMP-dependent protein kinase.  |  Keicher, C., et al. 2004. Biochem Biophys Res Commun. 324: 308-16. PMID: 15465019
5. Silencing of LASP-1 influences zyxin localization, inhibits proliferation and reduces migration in breast cancer cells.  |  Grunewald, TG., et al. 2006. Exp Cell Res. 312: 974-82. PMID: 16430883
6. Nuclear localization and cytosolic overexpression of LASP-1 correlates with tumor size and nodal-positivity of human breast carcinoma.  |  Grunewald, TG., et al. 2007. BMC Cancer. 7: 198. PMID: 17956604
7. Lasp-1 (MLN 50) defines a new LIM protein subfamily characterized by the association of LIM and SH3 domains.  |  Tomasetto, C., et al. 1995. FEBS Lett. 373: 245-9. PMID: 7589475
8. Chromosomal assignment and expression pattern of the murine Lasp-1 gene.  |  Schreiber, V., et al. 1998. Gene. 207: 171-5. PMID: 9511759

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-LASP-1 Antibody (G-7) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-LASP-1 Antibody (G-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

LASP-1 Antibody (G-7) Specifications

Alternate Product Name: LASP-1

Product Clone Name: G-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 176-205

Epitope Target: LASP-1

Epitope Species: human

Product Reactivity/Detection: LASP-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for LASP-1 Antibody (G-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin