LARS2 Antibody (G-9): sc-514454
- LARS2 Antibody (G-9) is a mouse monoclonal IgG1 κ LARS2 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 217-232 within an internal region of LARS2 of human origin
- LARS2 Antibody (G-9) is recommended for detection of LARS2 of human origin by WB, IP, IF and ELISA
- Anti-LARS2 Antibody (G-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for LARS2 Antibody (G-9) for WB applications. These reagents are now offered in bundles with LARS2 Antibody (G-9) (see ordering information below).
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LARS2 Antibody (G-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects LARS2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). LARS2 Antibody (G-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. LARS2, also known as leucyl-tRNA synthetase 2, mitochondrial, is a 903 amino acid protein that plays a crucial role in protein synthesis by catalyzing the aminoacylation of leucine to tRNA(Leu) through a two-step reaction. This process begins with the activation of leucine by LARS2 in the presence of ATP, forming an adenylate intermediate that subsequently transfers the activated leucine to the 3′-end of the target tRNA. The specific sequence and orientation of the variable loop in tRNA(Leu) are essential for interaction with LARS2, highlighting the importance of this protein′s structure in ensuring accurate protein synthesis. Dysregulation of LARS2 has been implicated in various neurological disorders, including bipolar disorder and schizophrenia, where LARS2 is thought to be over-expressed in an attempt to facilitate the aminoacylation of a mutated form of tRNA(Leu). Additionally, LARS2′s upregulation has been associated with diabetes, suggesting a potential role in glucose intolerance.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
LARS2 Antibody (G-9) References:
- Mitochondrial DNA 3243A>G mutation and increased expression of LARS2 gene in the brains of patients with bipolar disorder and schizophrenia. | Munakata, K., et al. 2005. Biol Psychiatry. 57: 525-32. PMID: 15737668
- Evidence that the mitochondrial leucyl tRNA synthetase (LARS2) gene represents a novel type 2 diabetes susceptibility gene. | 't Hart, LM., et al. 2005. Diabetes. 54: 1892-5. PMID: 15919814
- Molecular and functional dissection of a putative RNA-binding region in yeast mitochondrial leucyl-tRNA synthetase. | Nawaz, MH., et al. 2007. J Mol Biol. 367: 384-94. PMID: 17270210
- Modulation of substrate specificity within the amino acid editing site of leucyl-tRNA synthetase. | Zhai, Y., et al. 2007. Biochemistry. 46: 3331-7. PMID: 17311409
- A single residue in leucyl-tRNA synthetase affecting amino acid specificity and tRNA aminoacylation. | Lue, SW. and Kelley, SO. 2007. Biochemistry. 46: 4466-72. PMID: 17378584
- The C-terminal domain of the archaeal leucyl-tRNA synthetase prevents misediting of isoleucyl-tRNA(Ile). | Fukunaga, R. and Yokoyama, S. 2007. Biochemistry. 46: 4985-96. PMID: 17407269
- Isolated CP1 domain of Escherichia coli leucyl-tRNA synthetase is dependent on flanking hinge motifs for amino acid editing activity. | Betha, AK., et al. 2007. Biochemistry. 46: 6258-67. PMID: 17474713
- A Flexible peptide tether controls accessibility of a unique C-terminal RNA-binding domain in leucyl-tRNA synthetases. | Hsu, JL. and Martinis, SA. 2008. J Mol Biol. 376: 482-91. PMID: 18155724
- Gene cloning, expression and purification of human mitochondrial tRNA(Leu(UUR)) and its mutant. | Han, W., et al. 2001. Sci China C Life Sci. 44: 113-20. PMID: 18726427
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LARS2 Antibody (G-9) | sc-514454 | 200 µg/ml | $322.00 | |||
LARS2 Antibody (G-9): m-IgG Fc BP-HRP Bundle | sc-531133 | 200 µg Ab; 10 µg BP | $361.00 | |||
LARS2 Antibody (G-9): m-IgGκ BP-HRP Bundle | sc-524733 | 200 µg Ab, 40 µg BP | $361.00 | |||
LARS2 Antibody (G-9): m-IgG1 BP-HRP Bundle | sc-544411 | 200 µg Ab; 20 µg BP | $361.00 | |||
LARS2 Antibody (G-9) AC | sc-514454 AC | 500 µg/ml, 25% agarose | $424.00 | |||
LARS2 Antibody (G-9) HRP | sc-514454 HRP | 200 µg/ml | $322.00 | |||
LARS2 Antibody (G-9) FITC | sc-514454 FITC | 200 µg/ml | $336.00 | |||
LARS2 Antibody (G-9) PE | sc-514454 PE | 200 µg/ml | $349.00 | |||
LARS2 Antibody (G-9) Alexa Fluor® 488 | sc-514454 AF488 | 200 µg/ml | $364.00 | |||
LARS2 Antibody (G-9) Alexa Fluor® 546 | sc-514454 AF546 | 200 µg/ml | $364.00 | |||
LARS2 Antibody (G-9) Alexa Fluor® 594 | sc-514454 AF594 | 200 µg/ml | $364.00 | |||
LARS2 Antibody (G-9) Alexa Fluor® 647 | sc-514454 AF647 | 200 µg/ml | $364.00 | |||
LARS2 Antibody (G-9) Alexa Fluor® 680 | sc-514454 AF680 | 200 µg/ml | $364.00 | |||
LARS2 Antibody (G-9) Alexa Fluor® 790 | sc-514454 AF790 | 200 µg/ml | $364.00 | |||
LARS2 (G-9) Neutralizing Peptide | sc-514454 P | 100 µg/0.5 ml | $69.00 |